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The role of sucrose in lysis buffer is for subcellular fractionation. It refers to a laboratory technique that uses differential centrifugation to separate the different components of the cell.

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9y ago
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13y ago

It is a phosphatase inhibitor along with sodium orthovanadate.

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10y ago

the sucrose produce osmotic pressure out of the red blood cells and so destroyed them.

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15y ago

Providing an osmotic shock to the cells

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Q: What is the role of sucrose in RBC lysis buffer?
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When isolating DNA from blood, white blood cells (WBC's) are the target. This is because RBC's do not contain a nucleus and therefore do not contain DNA. The function of the lysis buffer is to help in the lysis (or breaking) of white blood cells. WBC's must first be lysed so that the DNA may be released from inside the cell.


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