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some orgs are too sensitive to be passed thru a flame. if the org cant withstand heat, it will be distorted and it will not yield an accurate representation of its phys properties in vivo when viewed under a mscope. Also, negative staining often involves nigrosine, India ink, etc which creates a thick layer which light cannot penetrate. So if heated, this layer cracks (many thin microscopic cracks) and you would not like that.
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Why steam heating is done in acid fast staining and not heat fixing?
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
How does one get bleach stains out of a gray sweater?
Bleach isn't a stain. Bleach removes the color. The only way to fix this is to bleach the whole sweater and then it will all be bleach colored.
If I get a stain on a table cloth that I can't bleach, does that pretty much render it ruined?
Unfortunately, if bleach doesn't fix it them nothing probably will. It may be time to get a new table cloth.
How do you get bleach off of shoes what product?
If the bleach has soaked in it will have removed colour from the material. So even removing the bleach if possible, will not fix the stain. You may need to re-dye if possible.
I was trying to fix a piece of broken to my gold chain with fevi quick but the white stains are left on that how do i remove it?
Dont remove it, paint over it with gold-colored paint.
Why is the size more accurate in a negative stain than a simple stain?
since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
What color is gram negative stain and gram positive stain if you forget to apply iodine?
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
What is the mordant reagent for?
to fix the color of the stain
What if you forgot to heat fix your slide?
If you forget to heat fix your slide your bacterial sample will be lost with the next wash step. So if you are doing a Gram stain when you add the crystal violet the liquid will mix with the bacteria, and when you wash later in the protocol the bacteria will wash away with the stains.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Why use methanol to fix smear during giemsa staining?
because giemsa stain is a mixture of methyl acetate Eosin and azure b. it doesnot contain any fixative that is why we use methanol to fix smear during giemsa stain other stain like lieshman contain acetyl free methyl alcohol as a fixative so it does not need to fix slide stain with lieshman stain.
What is meant by a polychromatic stain?
methods that fix different colors on different components of cells.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
How do you fix the heat in 2002 Chevy Impala?
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