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some orgs are too sensitive to be passed thru a flame. if the org cant withstand heat, it will be distorted and it will not yield an accurate representation of its phys properties in vivo when viewed under a mscope. Also, negative staining often involves nigrosine, India ink, etc which creates a thick layer which light cannot penetrate. So if heated, this layer cracks (many thin microscopic cracks) and you would not like that.
What else can I help you with?
Is iodine a basic stain?
No, iodine is not a basic stain. Iodine is commonly used in Gram staining to identify bacteria as either Gram-positive or Gram-negative based on their cell wall composition. It acts as a mordant in the staining process and helps to fix the crystal violet stain in Gram staining.
If I get a stain on a table cloth that I can't bleach, does that pretty much render it ruined?
Not necessarily. You can try using a stain remover or a color-safe bleach alternative to treat the stain. If that doesn't work, consider hiding the stain creatively with a centerpiece or table runner.
Why steam heating is done in acid fast staining and not heat fixing?
Actually, both methods are used during the staining procedure (steam & heat fix). Initially, the organism is heat fixed to the slide to prevent the organism from being washed off during subsequent steps. Later in the procedure, the slide with the heat fixed organism is steamed to make the cell wall a little more penetrable - allowing the stain to enter the cell wall.
How do you fix yellow bleach stains?
Unfortunately, yellow bleach stains are permanent as the bleach has altered the color of the fabric. You can try masking the stain with fabric dye or fabric markers, or consider adding a decorative patch over the stained area. Additionally, you can try using a color remover to lighten the entire garment to make the stain less noticeable.
You accidentally poured bleach on some black clothes - what can you do?
Accidentally pouring black on some black clothes will of course leave you with a variety of bleached spots on the clothing. The best thing that you can do to fix your clothes after this happens is to buy some dye and dye the clothing.
How can I fix a heat stain on wood?
To fix a heat stain on wood, you can try using a cloth soaked in a mixture of equal parts vinegar and olive oil. Rub the mixture onto the stain in a circular motion, then wipe it off with a clean cloth. Repeat as needed until the stain is removed.
Why is the size more accurate in a negative stain than a simple stain?
In negative staining, the stain outlines the outer edge of the cell, providing a more precise measurement of cell size. In contrast, simple stains can penetrate the cell membrane, making it difficult to accurately determine the cell boundary and thus its size. Negative staining is a better method for assessing size because it highlights the silhouette of the cell.
How can I fix a heat stain on my wood table?
To fix a heat stain on a wood table, try placing a cloth over the stain and using a warm iron to gently heat the area. This may help the stain to lift off the wood. Alternatively, you can try using a mixture of baking soda and toothpaste to gently scrub the stain away. Remember to always test any method on a small, inconspicuous area of the table first to ensure it does not cause further damage.
How can I fix a heat stain on a wood table?
To fix a heat stain on a wood table, you can try using a mixture of equal parts vinegar and olive oil. Apply the mixture to the stain and let it sit for a few hours before wiping it off. If the stain persists, you can also try using a mixture of baking soda and water to gently scrub the stain. Remember to always test any solution on a small, inconspicuous area of the table first to ensure it does not cause further damage.
What color is gram negative stain and gram positive stain if you forget to apply iodine?
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
How can I effectively fix heat stains on a wood table?
To effectively fix heat stains on a wood table, you can try using a mixture of equal parts white vinegar and olive oil. Apply the mixture to the stain and let it sit for a few hours before wiping it off. You can also try using a hairdryer on a low setting to gently heat the area and help the stain fade. If these methods don't work, consider refinishing or sanding the table to remove the stain completely.
How can I fix uneven stain on my project?
To fix uneven stain on your project, you can sand down the stained area to remove excess stain, then reapply the stain evenly across the entire surface for a consistent finish. Make sure to follow the manufacturer's instructions for the stain you are using.
Is iodine a basic stain?
No, iodine is not a basic stain. Iodine is commonly used in Gram staining to identify bacteria as either Gram-positive or Gram-negative based on their cell wall composition. It acts as a mordant in the staining process and helps to fix the crystal violet stain in Gram staining.
Why is my stain blotchy and how can I fix it?
A blotchy stain can occur due to uneven absorption of the stain into the wood. To fix it, you can try sanding the surface evenly, applying a wood conditioner before staining, and using a gel stain for more even coverage.
What is the appearance of gram negative bacteria after addition of mordant in the gram stain?
After the addition of mordant in the Gram stain, gram-negative bacteria will appear red or pink under the microscope. This is because the mordant helps to fix the crystal violet stain to the cell wall of gram-positive bacteria, making it harder for the decolorizer to remove it from the thicker peptidoglycan layer of gram-positive bacteria.
How can I fix uneven stain on wood?
To fix uneven stain on wood, you can sand down the surface to remove the existing stain, then reapply the stain evenly using a brush or cloth. Make sure to follow the manufacturer's instructions for the stain and allow it to dry completely before applying a protective finish.
