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To detect hydrogen sulfide production, a heavy metal salt containing ferrous iron is added to a nutrient culture medium, when hydrogen sulfide is produced the sulfide reacts with the metal salt producing the black precipitate.

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11y ago
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12y ago

The FeS appears as a black precipitate in the butt (or bottom) of the test tube.

The iron, Fe combines with H2S to form FeS (ferrous sulfide) a black compound. This will turn the butt (or bottom) of test tube black. Thus, a black butt indicates an H2S production.

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14y ago

Because black coloured FeS is heavier than water and can not float on the surface.

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Q: Why look for black precipitate FeS in the bottom instead of on the surface during a test with H2S in aqueous solution?
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