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7.4 ml of 0.2 molar acetic acid and17.6 ml of 0.2 molar sodium acetate made up with water to a total volume of 100 ml will have a pH of 5. The total acetate concentration (that is, acetate ions plus non-disocciated acetic acid) will be 50 mM. However, the concentration of acetate ions will be lower than 50 mM, but its value could be calculated with the Henderson-Hasselbalch equation. This is derived from the following source: @INCOLLECTION{Gomori1955, author = {G. Gomori}, title = {Preparation of buffers for use in enzyme studies}, booktitle = {Preparation and assay of enzymes}, publisher = {Academic Press, Inc, New York}, year = {1955}, editor = {Sidney .P. Colowick and Nathan.O. Kaplan}, volume = {I}, series = {Methods in enzymology}, chapter = {16}, pages = {138--146}, keywords = {buffers}, url = {http://microscopy.berkeley.edu/Resources/instruction/buffers.html}, } The acetate buffers table in Chapter 10 of the same Volume may also be helpful.
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∙ 16y ago
Wiki User
∙ 10y agoAcetate Buffer
Stock Solutions
A: 0.2 M solution of acetic acid (11.55 ml in I000 ml)
B: 0.2 M solution of sodium acetate (16.4 g of C2H302Na or 27.2 g of
C2H302Na" 3H20 in 1000 ml)
x ml of A + y ml of B, diluted to a total of 100 ml:
x y pH
46.3 3.7 3.6
44.0 6.0 3.8
41.0 9.0 4.0
36.8 13.2 4.2
30.5 19.5 4.4
25.5 24.5 4.6
14.8 35.2 5.0
10.5 39.5 5.2
8.8 41.2 5.4
4.8 45.2 5.6
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If 6.73g of Na2CO3 is dissolved in enough water to make 250ml of solution what is the molarity of the sodium carbonate what are the molar concentrations of the Na plus and CO32- ions?
6.73g (1 mole of Na2CO3/106g) = 0.063mol/0.25L = 0.25M Na2CO3 2 moles of Na+ x 0.25M = 0.5M Na+ 1 mole of CO32- x 0.25M = 0.25M CO3
Calculate the volume of solution needed to dissolve 0.25 moles NaCl to make a 3.0M solution?
3.00 M, or 3 moles per (L) "liter" calls for having 3 moles per liter of the solution. The question asks how many moles must be in 250ml of a solution that has 3 moles per Liter. You must ask yourself what percent of 1 Liter is 250mls? Since there are a thousand ml in one liter, (1000ml=1L), then 250ml is exactly 25% of a Liter, or .25L. So, 250ml can only hold 25% of the 3.00 Molarity. Meaning that you multiply 3 x .25 and get .75 moles.
How many moles of hydroxide ion are there in a 250 mL sample of 5.00 M solution of sodium hydroxide?
n=c/v n=3M/.25L n=12 mol m=Mxn m=58.443 g/mol x 12 mol m=701.3 g n= mol c=concentration v=volume m=mass M= molar mass Tylerops: I don't agree with this answer. Molarity is defined as Moles/Liters. In other words Molarity is the concentration of a solution. In the above n= Concentration / Liters. That is equal to saying Moles=(Moles/liters)/ Liters. In the above question the concentration is (3 moles/ liter), or 3M. Plus, how can it be possible to have 12 moles in 250ml when you only have 3 moles in each liter of the original solution? Correct ANSWER: 3.00 M, or 3 moles per (L) "liter" calls for having 3 moles per liter of the solution. The question asks how many moles must be in 250ml of a solution that has 3 moles per Liter. You must ask yourself what percent of 1 Liter is 250mls? Since there are a thousand ml in one liter, (1000ml=1L), then 250ml is exactly 25% of a Liter, or .25L. So, 250ml can only hold 25% of the 3.00 Molarity. Meaning that you multiply 3 x .25 and get .75 moles. 58.443g/molNaCl x .75 moles = FINAL ANSWER 43.83225g NaCl, Sig Fig, 43.83gNaCl
How do you prepare a 500mL of 1M of NaCl solutuion?
If you need to make just 100mL, then you need 1 tenth of a liter that is 5M. If you were to make 1L of 5 molar NaCl, you would need 5 times the molar mass of NaCl (58.44g/mol) dissolved in 1L of water. Thus for 1L of a 5M solution you need 5 * 58.44g, or 292.2 grams of NaCl. However, since we only want 100mL, which is 1/10 of a Liter, we also only need 1/10 the amount of NaCl, or 292.2 / 10, which is 29.22g. So, measure out 29.22g NaCl, and dissolve completly in a volume less than 100mL, say 80mL, then bring the final volume up to 100mL. You now have 100mL of a 5M NaCl solution.
What is the oxygen demand for a human?
In Rest a normal human (65 Kgs Body Weight) will need 250ml/min. of oxygen.Depending on the activity the body might consume 15 times as much oxygen as it needs in rest.
How to prepare 250ml of a 2m solution of potassium chloride?
111.72
How do you prepare 0.25N sulphuric acid from 2N sulphuric acid?
You should dilute it 8 times.So get 250ml of 2N solution and add to 1L and 750ml of water.
How much is 250 ml of milk?
250ml of milk = 250ml of milk 250ml of milk = roughly 256g of milk 250ml of milk = roughly 1 cup plus 2/3 of a tablespoon
How do you standardize 0.05M zinc chloride?
take 25ml of zncl2solution in a 250ml conicalflskand add 4gms of ammonium acetate and add 25ml of distilled water,xenlyl orange indicater sufficient hexamine end point is paleyellowcolour is produced
How do you prepare cultured sour cream?
Add to 250ml of pasteurised cream 15ml of lemon juice, stir to thicken. For a more sour taste simply add more juice
Is 1 mesuring cup 250ml?
It varies from 226 to 250ml.
What is 100GRAMS divided by 250ML?
0.4
How many 250ml in 1.89 liter?
There are 7.56 250ml units in 1.89 liters.
How many fluid ounces equal 250ml?
250mL is about 8.45 US fluid ounces.
How many 250ml bottles in 1 gallon?
About 15.1 250mL per US gallon.
Does a mug hold 250ml of water?
250ml of water equals 8.45 US fluid ounces, so it is possible that a mug could hold 250ml, depending on the size of the mug of course.
What is mass of 1 cup milk?
One official cup is 250ml. One cup of milk is 250ml.
