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Like proteins, DNA and RNA will unravel and lose its shape because the chemical bonds holding it together will break apart.
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Why sodium iodide for DNA elution?
its used as its a chaotropic salt..dat is it melts the agarose piece containing the DNA fragment when incubated at 45-55 degress Celsius
What is use of ethanol in DNA isolation?
Ethanol is used to precipitate the DNA. I.e. to bring the DNA out of solution. Precipitated DNA is then spun down and re suspended in the appropriate buffer that is suitable for sample storage
What is the chemical composition of chromatin?
Chromatin is the nucleoprotein material of chromosomes. It is made up of DNA attached to a protein structure, together with chromosomal RNA.
What happens when T2 phages are grown in the presence of radioactive phosphorus?
The bacterial proteins will become radioactive
What is the function of ammonium acetate in DNA extraction?
ammonium acetae use to percipitate DNA from water.
What is roll of calcium chloride in making competent cells in transformation?
The addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to Lipopolysaccharide. Positively charged calcium ions attract both the negatively charged DNA backbone and the negatively charged groups in the Lipopolysaccharide inner core. The plasmid DNA can then pass into the cell upon heat shock, where cells are cooled to a low temperature (+4 degrees Celsius) and then heated to a high temperature (+42 degrees Celsius) for a short time.
Why is chromosomal DNA stored at 4 degree Celsius?
DNA is best stored at 4 degrees Celsius because anything colder may cause extensive single and double strand breaks.
What is the steps in PCR?
1. Denaturation (separation of two strands of DNA by temperatures of around 94 to 98 degrees Celsius)2. Annealing (binding of DNA primer to the separated strands. Occurs at 50 to 65 degrees Celsius, which is lower than the optimal temperature of the DNA polymerases)3. Elongation (elongation of the strands using the DNA primer with heat-stable DNA polymerases, most frequently Taq (Thermus aquaticus) or Pfu (Pyrococcus furiosus) polymerases. Occurs at over 70 degrees Celsius)
Role of mgcl2 in competent cell preparation?
The addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to Lipopolysaccharide. Positively charged calcium ions attract both the negatively charged DNA backboneand the negatively charged groups in the Lipopolysaccharide inner core. The plasmid DNA can then pass into the cell upon heat shock, where cells are cooled to a low temperature (+4 degrees Celsius) and then heated to a high temperature (+42 degrees Celsius) for a short time.
Would chromosomal DNA spool if it was heated to 100 degrees?
If heated to a hundred degrees, chromosomal DNA would denature. Meaning the it would come apart and the complementary DNA strands would separate. One way to get DNA to spool (around a glass rod for example) is to remove it from the cell and precipitate it in solution. This can be done with with help of sodium chloride and isoamyl alcohol.
Why when extracting DNA from onion cells why is the mixture heated to 60 degrees for 15 minutes?
so as to kill the cell
How do you calculate genotype frequency?
particular DNA segment. Geno typing is the process of identifying an individuals genotype and there are a variety of methods for accomplishing this. One of the most common genotype techniques in polymerase chain reaction (PCR), which allows the analysis of very small samples due to its ability to make multiple copies of the DNA fragments.Difficulty: ModerateInstructions1Initialize the sample for polymerases that require a hot start. The sample is typically heated to about 95 degrees Celsius for about 5 minutes. Polymerases that are more thermos table may be heated to greater temperatures.2Denature the DNA fragments. Heat the sample to about 96 degrees Celsius for up to 30 seconds. This will disrupt the hydrogen bonds that join the two halves of the DNA fragment, causing the templates to separate from the primers.3Anneal the DNA templates and primers. Lower the temperature to the 50 to 65 degree Celsius range for about 30 seconds. This will allow hydrogen bonds to form between primers and templates that match each other very closely.4Elongate the primer strands. Raise the temperature to the 75 to 80 degree Celsius range and allow the Taq polymerase to begin synthesizing the primer strands. This can occur at an exponential rate under ideal circumstances.5Repeat steps 2, 3 and 4 approximately 30 times. After the last cycle is completed, a final step holds the sample at about 72 degrees for about 10 minutes to ensure the reaction has completed. The sample can then be stored at about 10 degrees Celsius.
What happens during the S phrase?
During the S stage DNA synthesis occurs. (copying of the DNA)
What happens when there is a letter change in DNA?
what happen when dna letters are changed
What happens in meiosis I that does not occur in meiosis I I?
DNA is copied.
What happens during DNA splitting?
You get two exact replica or the photocopies of DNA during splitting of DNA.
What happens when a DNA molecule unzips?
DNA splits, and mRNA and tRNA are there to create new strands for the new replicated DNA strand. This is what happens prior to mitosis in cell division.
