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The addition of calcium chloride to a cell suspension promotes the binding of plasmid DNA to Lipopolysaccharide. Positively charged calcium ions attract both the negatively charged DNA backboneand the negatively charged groups in the Lipopolysaccharide inner core. The plasmid DNA can then pass into the cell upon heat shock, where cells are cooled to a low temperature (+4 degrees Celsius) and then heated to a high temperature (+42 degrees Celsius) for a short time.
Wiki User
∙ 12y ago
Wiki User
∙ 11y agobecause mgcl2 is permeable to dna and promotes binding of e.coli to cell wall..
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What the meaning of cell pointers in Microsoft Excel?
A cell pointer in excel is just the cell where you point the cursor in which its row and column can be seen is called a cell pointer.
What are the steps in fermentation?
fermentation process mainly divided into 3 steps 1)Upstream 2)Fermentation 3)Down stream in up stream- strain selection, isolation,preservation, media preparation, innoculum preparation in down stream process- filtration, cell distruption,protein purification, lyophilization and paking
The place in a spreadsheet where a row and a column intersect is called a?
A cell. In the periodic table an element fills that cell.
What is wet cell weight?
The mass of a cell which has not been dehydrated.
Would a battery made out of lemon and two metals be a wet cell or a dry cell battery?
wet cell battery
Role of mgcl2 in DNA isolation?
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
Why use glycerol in competent cell preparation?
Glycerol is a cryo preservative gives density for samples to settle in well
Mechanism of cacl2 in competent cell preparation?
competence is the phenomenon exhibited by the cell during the particular stage of cell cycle by bacteria....it is log phase. During this stage the cell can accept any foreign DNA to enter in to it...so cell are grown upto the log phase or mid log phase and then transformed with the plasmid DNA
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
The process of a cell's nucleus replicating and dividing in preparation for the division of the cell is called?
mitosis
What does the cell do during G2?
during G2 of interphase there is cell growth and preparation for cell division, the cell does its job during G2
During which phase of cell reproduction does cell growth occur?
interphase- in preparation for cell division it increases its size. (cell growth occurs)
What role does RNAs play in the cell?
RNA plays a huge role in cell life.
What plays a role in cell division?
Nuclear division plays a role in cell division.
What substance is used to arrest a cell in metaphase for karyotype preparation?
Colchicines
Interphase preparation for didision?
In the inter-phase preparation for division stage, the cell increases it's own size and copies it's own DNA. The inter-phase is referred to as the living phase of the cell and where the cell performs all of it's duties.
What cell part plays a role in diffusion?
The plasma membrane of a cell plays a role in diffusion.
