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Why is NSS or 0.85 percent NaCl solution used in emulsifying the bacterial smear?
Wiki User
∙ 10y ago
0.85% nacl sol or normal saline solution is used as it is an isotonic solution which will emmulsify bacterial cells and dont cause any injury ( injury due to osmotic gradient in which cell can shrink or swell/burst ). solution upto 0.98% can be used.
Wiki User
∙ 10y ago
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What would be the result of overheating the bacterial smear?
your sample can destroy the bacteria and it will appear shapeless or enlarged when you overheat the bacterial smear
What are the characteristics of good bacterial smear?
no thing
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
Is a bacterial infection test the same as a chlamydia test?
Testing for chlamydia is very specific. A regular bacterial culture or wet smear will not detect chlamydia.
Why does the physician take a Pap smear?
Such swab tests are used to check for gonorrhea and chlamydia, or bacterial vaginitis, which is a bacterial infection resulting in inflammation of the vagina.
What is the function of methanol in the wright's stain solution?
to fixed the blood smear
What is a common mistake made when preparing a bacterial smear that results in large aggregates of bacteria piled on top of one another?
This is often the result of using far too many bacteria. You only need to take a very small loop from the bacterial culture to make a clear smear.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
How many percent that a woman can get pregnant after a pap smear?
Pap smears do not affect fertility. The same chance of pregnancy exists before or after a pap smear.
How do you examine live bacteria microscopically?
You observe bacterial smear using oil immersion lens with a magnification of 100x.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
