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Is dh5 alpha srain of E. coli is dam negative?
Yes, the DH5 alpha strain of E. coli is dam negative. This means that it lacks the Dam methylase enzyme responsible for DNA adenine methylation. DH5 alpha strains are commonly used in molecular biology research due to their ease of transformation and lack of methylation.
What is the significance of E. coli DH5 alpha?
E. Coli DH5 alpha cells are mainly of value to scientific research. They are frequently used for transformation of plasmid DNA, which is a research tool for amplifying the amount of plasmids. One can insert a gene of interest into a plasmid but the yield of this process is limited. If you were then to insert this plasmid into a living cell, your plasmid would be copied with every division of that cell. Bacteria normally host plasmids and are not too picky about it so one more is usually not a problem. Since bacteria like E. coli are really easy to grow, maintain and store, the DH5 alpha strain is perfect. As far as technical details about the strain go: DH5 alpha have been made deficient in some genes, which protects foreign DNA for instance. endA1 gene in DH5 alfa is modified (mutated) so that the intracellular endonuclease it code is inactive that degrades plasmid DNA in many preparations
What is the difference between DH5 alpha and BL21 with reference to cloning?
DH5 alpha is a common cloning strain used for general cloning purposes and is known for its high transformation efficiency. BL21 is an expression strain commonly used for protein production due to its use of T7 RNA polymerase for high-level protein expression. The main difference is in their applications, with DH5 alpha used for cloning and BL21 used for protein expression.
What are the key characteristics and applications of the DH5 alpha strain in molecular biology research?
The DH5 alpha strain is commonly used in molecular biology research due to its high transformation efficiency, making it easy to introduce foreign DNA. It is also known for its ability to maintain plasmids well and its sensitivity to antibiotic selection. Researchers often use this strain for cloning, gene expression studies, and protein production.
You have taken two control.One with LB media only and other with LB plus kanamycin.LB plus kanamycin showed growth upon incubation. what could be the reason?
This means the bacteria that you are handling is resistant to kanamycin and can therefore grow in the presence of this antibiotic. You have to check if there are colonies on the control plate containing only LB medium. If there are colonies, then this reasoning is valid. A possible reason why bacteria can grow in the presence of kanamycin is they could have a plasmid that is conferring antibiotic resistance. However, this is only a hypothesis and further investigation (like plasmid isolation) is required to confirm it.
Alternative to use of kanamycin for surgical irrigation-thanks?
I dunno, we are in the same situation with the temporary discontinuation of Kanamycin. We will most likely switch to Amikacin.
What is kanamycin sulfate?
It is an oral anti-infective
Is kanamycin used in neospora caninum tacyzoite culture?
No
What does kanamycin target?
Kanamycin targets bacterial ribosomes by inhibiting protein synthesis, leading to the disruption of bacterial growth and ultimately cell death.
Why does alpha amanitin not inhibit the activity of RNA polymerase II of members of the Amanita genus of mushrooms?
Members of the Amanita genus of mushrooms, including the species that produce alpha-amanitin, have evolved RNA polymerase II variants that are resistant to the inhibitory effects of alpha-amanitin. These resistant RNA polymerase II variants have subtle structural differences that prevent alpha-amanitin from binding effectively and inhibiting their activity. This adaptation allows the mushrooms to continue transcription even in the presence of alpha-amanitin, which is toxic to most other organisms.
Name a medicine that starts with the letter k?
Kanamycin Sulfate is all I can think of...
What is the significance of Ecoli DH5 alpha?
This strain of E. coli has many mutations that make it useful for transformation. Its genotype is dlacZ DeltaM15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rK-mK+) supE44 thi-1 gyrA96 relA1 but the most useful of these mutations are: The lacZDelta M15 mutation allows for blue/white screening for recombinant cells. recA1 mutation reduces homologous recombination for a more stable insert. endA1 mutation reduces endonuclease digestion of plasmid for higher plasmid yield. hsdR17(rK-mK+) for reduced activity of EcoK restriction enzyme.
