- Human cells,from the individual who will be cloned.
- Media in which these human cells will grow and divide.
(breeding oven)
- Media in which cells will stop dividing, and enter a state
of "quiescence" without dying.(freezer)
- Laboratory supplies: Incubator, Sterile Hood, petri dishes,
microscopes, and tools capable of removing and implanting
cellular organelles,from one cell to another.
- Unfertilized human egg cells.
- Media in which the fertilized eggs can grow and
step 2Commence cloning!This first step is easy: You only need to grow the cells until you have the amount you want.The second step isn't hard either: Transfer the cells to the freezer. This should allow the cells to live, but they should stop dividing and enter quiescence.
The third step is a hard one for the unexperienced cloner: When the cultured cells are in the quiescent state, get an unfertilized human egg cell. Remove the nucleus from this egg cell. Try to minimize damage done to this cell and discard the nucleus, because this has become useless now.
The forth step is a rather difficult one too:
Take one of the quiescent cells in it's entirely, and implant it inside the coat around the egg (known as the zona pellucida, if you really want to know) next to the egg itself.
The fifth step isn't as hard as step 3 or 4: Electroshock the egg. The electroshock induces the fusion of the two cells, so you should be able to tell when you've electroshocked enough just by looking at the cells.
The sixth (and last)step: Repeat the last three steps as necessary until you have enough clones. Expect a lot of them not to survive because of cellular damage and other mishaps. Allow the embryos to grow and divide a few times in Human Egg Cell growth media. Now implant the embryos in human mothers where they can be carried to term, and born normally. After a few months (approximately 9) you have your own child :) divide
step 3Legal stuffCloning is illegal in the following countries:*USA
*UK
*All of the European Union
*the UN is against cloning, but doesn't force their members to
abolish it
...
So it is not allowed to make a real clone (which renders this Instructable useless XD).
When you really want a child, I suggest you find yourself a wife/man and do it the traditional way (which I personally find to be more fun:P)
This instructable is made for entertainment only, please don't try this at home.
nope, if what i understand is right, then no they don't.
clones have to be created in a lab in order to make the diffusion with both sperm and egg.
Cloning can make all the descendants of a particular line susceptible to the same possible defects and mutations.
Clones are produced by asexual reproduction. There's only one parent.
Cloning VectorsVectorMaximum Insert sizeApprox. No. of clones required in libraryAdvantages?Disadvantages?lambda20 kb5 x 105easy to construct libraries,relatively stable insertsmany clones requiredhard to prepare DNA from clonescosmid45 kb2 x 105easy to construct librarieseasy to prepare DNA from clonesnot always stableYAC1 Mb104few clones requiredvery prone to rearrangement,difficult to constructBAC> 500 kb5 x 104few clones requiredvery stablesingle copy origin of replication therefore harder to prepare DNA
No they are Yamaha clones but are made in China
Master sifuds he gave permishin for the clones to be made if he hadn't the clones wouldn't have fallan under control of sidois dus not cuausing all the jedi to die under the clones DC-15's
i am pretty sure they are made using stem cells
According to Wookieepedia, the order for the clones was made secretly by a Jedi Master named Sifo-Dyas. The order was funded by Count Dooku.
Clones have been made in many countries.
A group of clones is typically referred to as a "clone army" or simply a "group of clones."
Clones? Last I checked where in 2009.
Generally, Cloning has no negative side. Clones can be programmed to obey their duties. But while the clones were being generated, they were made to respond to Order 66, which means- "destroy the Jedi"
Tigernach of Clones died in 549.
Attack of the Clones was released on May 16, 2002.
In the year 3024.
Outsmart The MBA Clones was created in 2008.