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What has the author Dannie G Hollis written?

Dannie G. Hollis has written: 'Gram-positive organisms' -- subject(s): Gram's stain, Bacteria


Why is Gram of gram stain written in capital letters?

The eponym Gram stain is capitalized because it is named after the Danish bacteriologist Hans Christian Gram, who developed the staining technique in 1884. Capitalizing the "G" in Gram distinguishes it as a proper noun associated with a specific individual.


How do you eliminate gram positive from mix with gram negative?

can this be done by using gram differential stain and then lysozme, washing with a mild detergent mechanically to release the dead debris of the lysed G (+) bacteria.


What is the most crucial step in the gram stain procedure?

The most critical step of gram staining is the decolorization step as crystal violet stain will be removed from both G+ve & G-ve cells if the decolorizing agent(e.g alchohol ) is left on too long.


Do you capitalize all three letters in god?

No, o nly the letter g. It should be God.


Function of crystal violet in gram stain?

Well if you stained with only crystal violet, then they would all be violet! If you do a Gram Stain the right way, you end up getting Gram positive being violet or purple and Gram Negative being red or pink.


How does thickness of peptidoglycan affect the outcome of gram stain?

For Gram Stain experiment. The gram-positive, g+, which stained purple in color (use Crystal violet) as the bacteria contain a thicker layer of peptidoglycan with no lipopolysaccharide and protein bound the outer membrane of the bacteria (bacteria A). The gram-negative, g-, which stained in red (if the safranin (red dye/counter stain is use) appear due to the bacteria (bacteria B) which lack of peptidoglycan and it is bound by a lipopolysaccharide and protein layer. The thicker the peptidoglycan, the more coloring agent (crystal violet ions, CV+) it can absorb. Hence, give more purple in color to the bacteria and this will be the gram-positive, g+ bacteria. On the other hands, bacteria B seem has thinner peptidoglycan thus it absorb less purple color from the CV+, and after decolourise (using alcohol) and stained with safranin (red dye), only the bacteria with lack of peptidoglycan change to red hence we can say bacteria B as gram-negative, g-. Hence the outcome is the bacteria with thicker peptidoglycan layer be the gram-positive, g+(purple color) while the thinner peptidoglycan layer be the gram-negative, g-. (red color)


How does thickness of peptidoglycan affect gram stain outcome?

For Gram Stain experiment. The gram-positive, g+, which stained purple in color (use Crystal violet) as the bacteria contain a thicker layer of peptidoglycan with no lipopolysaccharide and protein bound the outer membrane of the bacteria (bacteria A). The gram-negative, g-, which stained in red (if the safranin (red dye/counter stain is use) appear due to the bacteria (bacteria B) which lack of peptidoglycan and it is bound by a lipopolysaccharide and protein layer. The thicker the peptidoglycan, the more coloring agent (crystal violet ions, CV+) it can absorb. Hence, give more purple in color to the bacteria and this will be the gram-positive, g+ bacteria. On the other hands, bacteria B seem has thinner peptidoglycan thus it absorb less purple color from the CV+, and after decolourise (using alcohol) and stained with safranin (red dye), only the bacteria with lack of peptidoglycan change to red hence we can say bacteria B as gram-negative, g-. Hence the outcome is the bacteria with thicker peptidoglycan layer be the gram-positive, g+(purple color) while the thinner peptidoglycan layer be the gram-negative, g-. (red color)


Would methylene blue stain be substituted for safranin as the counter stain in this procedure?

Methylene blue is a stain used in microbiology. The dye attaches to the object, which allows what would normally be fairly see-through to be visible to the microscope.


What is the abbreviation for grams?

The abbreviation for gram is gm. and the symbol is g. Gram is short g.


What stands for gram in measuring?

gram or g stands for gram.


Do you need to capitalize the g in Grandpa?

yes you do