Blender
They tore of the surface of the surfaces of the bacteria with the help of a blender.
Hershey and Chase discovered that after the P-labeled phages infected the bacteria, most of the radioactive phosphorus was found in the viral DNA, while little to no radioactive sulfur (used to label protein) was found. This confirmed that genetic material, not protein, is responsible for heredity in viruses.
Hershey and Chase confirmed that DNA is the genetic material through their famous experiment using bacteriophages, which are viruses that infect bacteria. They labeled the DNA of one batch of phages with radioactive phosphorus and the protein coat of another batch with radioactive sulfur. After allowing the phages to infect bacterial cells, they found that only the labeled DNA entered the bacteria and directed the production of new phages, while the protein remained outside. This demonstrated that DNA, not protein, carries the genetic information necessary for viral replication.
Hershey and Chase knew that the particular phage they worked with has two basic components: DNA on the inside, coated with protein on the outside. In their expt theylabelled phages with radioisotopes . For one batch of infecting phages, they used a radioactive isotope of sulfur to label only the phages' protein coats.In another batch of phages, they used a radioactive isotope of phosphorus to label only DNA. Next, they allowed each batch of phages to infect separate cultures of nonradioactive bacterial cells. They then whirled each culture in a blender to shake loose any parts of the phages that remained outside the bacterial cells. Result of expt. confirmed that only DNA of phage entered bacteria .
The genetic mateial is made of DNA and not of proteins
filters
According to my bio book, I think it's Alfred Hershey and Martha Chase, in the HERSHEY-CHASE experiment.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Hershey and Chase observed that DNA is the identity of the hereditary material in phage T2. Hershey and Chase used radioactive labels to mark the protein of the bacteriaphage in one experiment and the DNA in another.
Their genetic material
Their genetic material
Alfred Hershey and Martha Chase conducted the famous Hershey-Chase experiment in 1952, which confirmed that DNA, not protein, is the genetic material in bacteriophages. They used radioactive labeling to trace the genetic material within the bacteriophages, providing evidence for the role of DNA in heredity.