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Bromphenol blue is a pH indicator that changes color depending on the acidity or alkalinity of a solution. In a more acidic environment (pH below 3.0), it appears yellow, while in neutral to alkaline conditions (pH above 4.6), it transitions to blue. This property makes it useful in various laboratory applications, including as a tracking dye in gel electrophoresis, where it helps visualize the migration of molecules based on their size and charge. Additionally, it can be used as a staining agent in biochemical assays.

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