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Why was Koch's recommendation for fixing and staining important for microbiology?
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Why was koch's recommemdation for fixing and staining important for microbiology?
Koch's recommendation for fixing and staining is important for microbiology even though all bacteria can be seen under the microscope, because staining and fixing is needed in order to see inside of a transparent bacteria.
What common sources of gram staining errors?
improper fixing
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
What would happen if no fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
What are the disadvantages of heat fixing?
One of the disadvantages of heating fixing is that it is very expensive. Secondly, it cannot be used in capsular staining.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
Why are nitrogen fixing bacteria so important to other organisms?
why are nitrogen fixing bacteria so important to other organism
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What would happen if no heat fixing were done?
If no heat fixing was done to a slide with a specimen on it, it would be rinsed off with the gram staining procedure. Heat fixing the specimen does kill specimen but it also locks it in place.
Which is the most abundant carbon-fixing enzyme on earth?
Which is the most important and abundant carbon-fixing enzyme on earth
Function of heat-fix in gram staining?
Heat fixing is done to kill the specimen; adhere the specimen to the slide; and alter the specimen so that they more readily accept stains.
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
