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By using darkfield or oblique lighting. The easiest is oblique. Just put a piece of tape over about half of the bottom lens of your condenser. Now instead of the light hitting the object from directly below the light bounces in from the side. This kind of gives a 3D effect to the transparent object.

Darkfield uses the same principle except that you block the center portion with a circle of opaque material. It's called a "stop". It stops the light from coming through the center of the lens and again, illuminates the object from the side.

A lot of basic microscopy books will have this information in them. Also there are a lot of microscopy sites out there. Microscopy-UK.org is one; also Olympus (olympusmicro.com) and Nikon (microscopyu.com) have some good sites.

Phloem

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14y ago

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