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Why do you use on or slide to be examined with the oil immersion objective?

If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.


What microscope objective is best to study bacteria?

A 100x oil immersion objective is best for studying bacteria under a microscope as it provides the highest magnification and resolution needed to observe bacterial morphology and structure in detail. Additionally, the use of oil immersion helps to reduce the refraction of light and improve image clarity.


What advantage does low power objective have over oil immersion objective?

A low power objective has a wider field of view and is easier to use because it doesn't require immersion oil. This makes it convenient for quickly scanning samples or observing larger structures on a slide without the need for oil immersion.


Why it is necessary to wipe off the immersion oil after using the oil immersion objective?

Leaving immersion oil on the objective can attract dust and debris, which can impact imaging quality and potentially damage the lens. In addition, it can cause the oil to harden and become difficult to remove, leading to maintenance issues. Cleaning the oil off after use ensures optimal performance and prolongs the lifespan of the objective.


Should you store the microscope with the oil immersion lens in position?

No, it is not recommended to store the microscope with the oil immersion lens in position. It can lead to the oil drying out and potentially damaging the lens or affecting the quality of future observations. It is best to clean the lens and remove excess oil after use before storing the microscope.


Which objective focuses closest to the slide when it is in focus?

The objective lens that focuses closest to the slide is 100x, it has the longest lens so the highest power. Be careful not to crack the slide and make sure you use oil if it is an oil immersion lens.


When would you need to use the oil immersion lens?

Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.


What is the magnification of objects observed through a 100X oil immersion objective with a 7.5X eyepiece?

The total magnification would be 750X (100X objective multiplied by 7.5X eyepiece). Oil immersion objectives are specifically designed for use with immersion oil to minimize light refraction and increase resolution when viewing specimens with high magnification.


Why should the oil immersion objective be cleaned immediately after use?

Cleaning the oil immersion objective immediately after use is important to prevent dried oil residue from accumulating and affecting image quality. It also helps to maintain the lens in good condition and prevent contamination between samples. Delaying cleaning can make it more difficult to remove the dried oil and may lead to damage or reduced performance of the objective.


How do you calculate the resolution on a light microscope?

Multiply the magnification of the ocular and objective lenses. For an example, an ocular lense with mag 10X and an objective lense with mag 40X would result in a total magnification of 400X.


What is the magnification of a microscope with a 10x ocular and a 95x oil-immersion objective?

950


Which of the objectives comes closest to a specimen and is most likely to break a slide if proper precautions are not taken?

The oil immersion objective comes closest to the specimen and is most likely to break a slide if proper precautions are not taken. Oil immersion objectives require the addition of immersion oil between the objective lens and the slide to improve resolution, and without the correct amount of oil or if the slide is not handled carefully, it can lead to damage or breakage of the slide.