by the help of hybridoma technology. single type of B cell which is sensitized with single epitope of antigen fysed with a myeloma cell. then that fused cell produce single type of antibody....that is monoclonal antibody.....
Monoclonal antibody
An agonisitic monoclonal antibody is an immunological term for a monoclonal antibody which attempts to boost the immune system in order to fight infection or cancer.
A monoclonal antibody is a highly specific antibody that has been formed in a laboratory setting. It is made up of clones from a hybrid cell made from a fusion of B-cells with a tumor cell.
Adalimumab is a therapeutic monoclonal antibody that is made from human proteins. It is produced using recombinant DNA technology in a laboratory setting.
Humira (generic name adalimumab) Is The First Human Monoclonal Antibody Approved For Rheumatoid Arthritis.
Monoclonal antibody therapy is administered through injections. How often it should be perfomed varies depending on what your doctor recommends.
Monoclonal antibody production can be seen as very valuable as it creates an insight into diagnosing in vitro anomalies. Monoclonal antibodies are derived from cloned cells and allow people to study in depth anomalies that occur in humans and in reproduction.
MoAb stands for "monoclonal antibody"
find the protein sequence in protein sequence data base.....then from the protein sequence u can find the antibody gene sequence...... u can also go for nucleotide sequencing....which will directly help u in getting the sequence.....u can check this in bioinformatics data base
Polyclonal antibody recognizes several epitopes on the target protein while monoclonal antibody recognizes only single epitope. Sometimes, monoclonal antibodies are not able to precipitate the antigen because the epitope might need to be exposed on the surface of the antigen to be recognized by the antibody. Since the epitope might be hidden and it's a single epitope that is recognized by the monoclonal antibody, the propability of the antibody to reconize the epitope is lower compared with the polyclonal antibody that recognizes several epitopes on the target protein.
Xolair is marketed as omalizumab. It is a monoclonal antibody used to treat allergic conditions such as asthma and chronic idiopathic urticaria by targeting and neutralizing immunoglobulin E (IgE).
Polyclonal antibody recognizes several epitopes on the target protein while monoclonal antibody recognizes only single epitope, hence monoclonal antibodies are more specific than polyclonal antibodies. However, sometimes MAbs are not able to precipitate the antigen because the epitope might need to be exposed on the surface of the antigen to be recognized by the antibody. Since some of the epitope might be hidden and it's a single epitope that is recognized by the monoclonal antibody, the propability of the antibody to reconize the epitope is lower compared with the polyclonal antibody that recognizes several epitopes on the target protein this is the reason for the tendency of polyclonal antibodies to have cross-reaction as compared to MAbs. by Victor S Gruezo Jr