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What is the function of the general transcription factor TFIID?
Wiki User
∙ 11y ago
TFIID recognizes the TATA box.
Wiki User
∙ 11y ago
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What does Transcription in eukaryotes require in addition to RNA polymerase?
General Transcription Factors (GTF). TFIID (binds to DNA first at TATA box), TFIIA, TFIIB, TFIIF, TFIIE and TFIIH.
Which is the protein involved in promoter recognition in eukaryotes?
TFIID
The correct order for the assembly of the eukaryotic transcription complex is?
Transcription factors bind in this order: D, A, B, F, E, H, J.RNA polymerase (usually RNA pol II for transcription fo protein coding genese) binds with TFIIF.DetailsInitially, TFIID binds to the TATA box of the DNA strand, if present, since the TBP is part of the TFIID complex. The shape of TBP causes large changes in the shape of the DNA molecule, unwinding it greatly. This allows TFIIA and then TFIIB (with TFIID) to bind to the promoter region, followed by TFIIF (similar to sigma factor in prokaryotes) with RNA polymerase II, then TFIIE, which enables the polymerase to move along the DNA easily, and TFIIH, which contains a helicase to unwind the DNA and an enzyme region to phosphorylate (initiating elongation) a region of the polymerase II enzyme known as the C terminal domain (which is unique to this form of polymerase II). This forms a complex known as the basal transcription apparatusOther transcription factors may also bind depending on the specific gene being transcribed. TBP binds specifically to the TATA box, and in the case of the large number of genes that do not use the TATA consensus sequence this will be replaced by that gene's alternative.For further information on the structure of the completed complex, I recommend the structure of the RNA polymerase II elongation complex to 3.3 angstroms as determined by Gnatt et al.
What could be a beneficial effect of a mutation?
gene mutation has many profound and long lasting effects. for starter mutation can change course of evolution. it also can create many heridatory deseases. it can make some desease cousing organisms to become drug resistant. gene mutation can create new species.
What are the steps involved in the process of transcription?
DNA unwinds/"unzips" as the Hydrogen Bonds Break.The free nucleotides of the RNA, pair with complementary DNA bases.RNA sugar-phosphate backbone forms. (by RNA Polymerase.)Hydrogen bonds of the untwisted RNA+DNA "ladder" break, freeing the new RNA
