The correct order for the assembly of the eukaryotic transcription complex is?

Answer 1

Transcription factors bind in this order: D, A, B, F, E, H, J.

RNA polymerase (usually RNA pol II for transcription fo protein coding genese) binds with TFIIF.

Details

Initially, TFIID binds to the TATA box of the DNA strand, if present, since the TBP is part of the TFIID complex. The shape of TBP causes large changes in the shape of the DNA molecule, unwinding it greatly. This allows TFIIA and then TFIIB (with TFIID) to bind to the promoter region, followed by TFIIF (similar to sigma factor in prokaryotes) with RNA polymerase II, then TFIIE, which enables the polymerase to move along the DNA easily, and TFIIH, which contains a helicase to unwind the DNA and an enzyme region to phosphorylate (initiating elongation) a region of the polymerase II enzyme known as the C terminal domain (which is unique to this form of polymerase II). This forms a complex known as the basal transcription apparatus

Other transcription factors may also bind depending on the specific gene being transcribed. TBP binds specifically to the TATA box, and in the case of the large number of genes that do not use the TATA consensus sequence this will be replaced by that gene's alternative.

For further information on the structure of the completed complex, I recommend the structure of the RNA polymerase II elongation complex to 3.3 angstroms as determined by Gnatt et al.

Answer 2

The assembly of the eukaryotic transcription complex begins with the binding of general transcription factors (e.g., TFIIA, TFIIB) to the promoter region, followed by the recruitment of RNA polymerase II and additional factors such as TFIID. This assembly process helps facilitate the initiation of transcription by forming a stable complex at the promoter site.