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What is the difference between Elisa direct, indirect, and sandwich assays in terms of their application and detection methods?

Elisa direct, indirect, and sandwich assays are all types of enzyme-linked immunosorbent assays used to detect specific molecules in a sample. In a direct Elisa assay, the target molecule is directly immobilized on the plate and detected using a labeled antibody that binds to it. In an indirect Elisa assay, the target molecule is immobilized on the plate and detected using a primary antibody that binds to it, followed by a labeled secondary antibody that binds to the primary antibody. In a sandwich Elisa assay, the target molecule is captured by a specific antibody immobilized on the plate, then detected using a labeled secondary antibody that binds to a different epitope on the target molecule. Each type of assay has its own advantages and limitations in terms of sensitivity, specificity, and ease of use, making them suitable for different applications in research and diagnostics.


What memory type is commonly used to detect and correct errors?

ecc


Why is the ELISA test so sensitive?

It has many reason that may be possible. 1. Stability of Elisa kit. 2. Type of Elisa kit ... Example. Rat IL-1 must use with cell rat .... but can use with the other depend cross reactivity of Elisa kit. 3. Right method will show good standard curve. (r2 almost 1 ) if not it's wrong. 4. The concentration used of treated in cell is not appropiate .


Softwares that detect type of motherboard?

There are a number of softwares and programs that are used to detect the type of the motherboard. The CPU-Z software is the most commonly used software fr this purpose.


Is an antibody a type of protein?

Yes, an antibody is a type of protein that is produced by the immune system to help fight off infections and diseases.


What is antibody and what you is the function?

Antibody is a type of protein produced by the immune system, its function is to fight antigens (bacteri, virus)


How many types of antigens can bind to a single antibody?

An antibody can typically bind to one specific type of antigen.


What is the antibody of the antigen AB?

Type AB blood doed not contain any antibodies. I does have Antigens A and B.


Which type of leukocyte is responsible for antibody production?

lymphocytes


How monoclonal antibody are produced?

by the help of hybridoma technology. single type of B cell which is sensitized with single epitope of antigen fysed with a myeloma cell. then that fused cell produce single type of antibody....that is monoclonal antibody.....


The type of antibody that is secreted first after the arrival of an antigen?

Lgm


What hypersensitivity reaction does not involve an antibody response?

Type IV