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The cell wall of bacteria is made peptidoglycan which consists of polysaccharide and peptide. Peptide crosslinks help hold the polysaccharide chains together. The bacterial enzyme D-alanyltranspeptidase is known to affect these crosslinks ans it binds to the D-Ala-D-Ala end of peptisdes.

The beta-lactam region of the antibiotics are similar to the D-Ala-D-Ala ends of peptisdes. They can hence inhibit D-analytranspeptidase by creating a enzyme intermediate which slowly hydrolyses the covalent acyl bond.

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