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Mary Queen of Scots was Elizabeth's first cousin once removed. Mary's grandmother was sister of Elizabeth's father Henry VIII. But they were not first cousins.

Elizabeth's first cousins were:

By her aunt Margaret Tudor:

James, Duke of Rothesay

Arthur, Duke of Rothesay

James V of Scotland (Mary Queen of Scot's father)

Alexander Stewart, Duke of Ross

Margaret Douglas, Countess of Lenno

By her aunt Mary Tudor:

Henry Brandon († 1522)

Frances Grey, Duchess of Suffolk

Eleanor Clifford, Countess of Cumberland

Henry Brandon, 1st Earl of Lincoln

By her aunt Mary Boleyn:

Henry Carey, 1st Baron Hunsdon

Catherine Carey, Lady Knollys

Anne Stafford

Edward Stafford

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