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What were Erwin Chargaff's 3 major accomplishments?
Erwin Chargaff is best known for formulating Chargaff's rules, which state that in DNA, the amount of adenine equals thymine, and the amount of cytosine equals guanine. This discovery was crucial in understanding the base pairing mechanisms of DNA. Additionally, he contributed to the understanding of the biochemical composition of nucleic acids and played a significant role in the development of molecular biology. His work laid important groundwork for the later discovery of the DNA double helix structure by Watson and Crick.
What key event happens during the s phase?
synthesis of DNA DNA Replication
What is DNA medicine?
DNA has largely replaced finger-prints for indentifying people. Or at least confirmed what finger-prints have led authorities to. Service people in the military today, all must now submit their DNA to the medical corps, so that in the event they become a casualty, they can be identified. DNA science has probably eliminated future "Unknown Soldiers." For detailed definitions of DNA, see site: DNA
Did an African American contribute to the discovery of DNA?
No, DNA was first isolated by the Swiss physician. Also there were no African Americans involved in any subsequent work on DNA leading to the understanding of it structure.
Are genes a length of DNA?
Yes, a gene is a complex length of DNA that is in our body. A gene is a length of DNA that codes for a living life form. Genes are found on chromosomes which are in the body nucleus.
What is the role of beta-mercaptoethanol in DNA extraction?
stabilization of phenol against oxidation
What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?
It is an antioxidant.
What is the role of NaCl in DNA precipitation?
roll of Na CL in DNA extraction
What is the Role of glycerol in DNA extraction?
Glycerol is sometimes added to DNA extraction buffers to increase the density of the solution, allowing DNA to precipitate more efficiently. It also helps stabilize DNA during extraction procedures by preventing degradation from nucleases.
Role of tris in extraction of DNA?
To give the solution buffering capacity.
Role of beta-mecaptoethanol in DNA isolation?
beta- merceptoethanol denatures the protein by breaking the sulphur bridges in it.
What role does EDTA play in DNA extraction processes?
EDTA is a chelating agent that helps to bind and remove metal ions that can degrade DNA during extraction processes. It helps to stabilize the DNA and prevent enzymatic degradation, allowing for a more efficient and successful extraction of DNA.
What is the role of mercaptoethanol in DNA isolation?
Mercapotoethanol is used as an antioxidant. Especially for polyphenolics which become insoluble when oxidised and tend to precipitate with the DNA. Sodium sulphite (1.2 g/L) does the same thing without the stink.
What is the role of ethanole in DNA isolation?
2-Mercaptoethanol is often included in extraction buffers designed for plant DNA extraction, because it is a strong reducing agent which can remove tannins and other polyphenols often present in the crude plant extract. It may also help to denature proteins by breaking disulphide bonds between cysteine residues.
What is the Role of calcium acetate in DNA extraction?
Calcium acetate is used in DNA extraction to neutralize the negative charge of DNA molecules, allowing them to aggregate and precipitate out of solution. This helps to separate DNA from other cellular components during the extraction process, making it easier to isolate pure DNA for downstream applications.
What is the role of 70 ethanol in DNA extraction?
70% ethanol is used in DNA extraction to wash and precipitate DNA from a sample. Ethanol helps to remove impurities and salts, allowing DNA to clump together and be easily separated from the rest of the sample. It also helps to preserve the integrity of the DNA during the extraction process.
What is the role of isopropanol in DNA extraction?
Isopropanol is used in DNA extraction to separate DNA from other cellular components. It helps to precipitate the DNA, causing it to clump together and separate from the rest of the solution. This allows for the isolation and purification of the DNA for further analysis.
