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At her birth: Catherine of Aragon's supporters, who thought her the bastard daughter of a harlot.

Later, her sister would become her enemy, because of Elizabeth's refusal to embrace catholocism and popularity with protestants (and the threat this presented to her unpopular reign).

After Elizabeth became queen; Some Catholics in England (though her relative tolerance meant most did not hate her), her cousin Mary of Scots and her supporters, Philips II increasingly, the pope, most of Catholic Europe.

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