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puc18-

plasmid of university of California 18

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What is the difference between Puc18 and Puc19?

The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.pUC18: LacZ HindIII PaeI ..... SacI EcoRIpUC19: Lacz EcoRI SacI ..... PaeI HindIII


Is PUC18 a common YAC true or false?

False


Puc18 is a vector or a plasmid?

PUC18 is a plasmid, specifically a commonly used cloning vector in molecular biology research. It is small in size and contains a multiple cloning site for inserting DNA fragments, as well as antibiotic resistance genes for selection in bacteria.


What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?

The enzyme produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18 is luciferase. This enzyme is responsible for the bioluminescent properties of animals like fireflies and glowworms. Cells transformed with plasmid lux will emit light in the presence of the substrate luciferin, whereas cells transformed with pUC18 will not.


Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?

The pUC18 plasmid contains the ampicillin resistance gene (ampR) which confers resistance to ampicillin. The Lux operon on the plasmid allows for bioluminescence production and acts as a reporter gene. Therefore, transformed cells that harbor both plasmids can grow in the presence of ampicillin due to pUC18 and express bioluminescence due to the Lux operon.


What is the abbreviation for an associate's degree?

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Standard Abbreviation: Ill.Postal Abbreviation: IL


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