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puc18-
plasmid of university of California 18
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What is the difference between Puc18 and Puc19?
The multiple cloning sites (MCS) in pUC18 and pUC19 is the difference - the MCS in pUC19 is reverse orientated to those of the pUC18.pUC18: LacZ HindIII PaeI ..... SacI EcoRIpUC19: Lacz EcoRI SacI ..... PaeI HindIII
Is PUC18 a common YAC true or false?
False
Puc18 is a vector or a plasmid?
PUC18 is a plasmid, specifically a commonly used cloning vector in molecular biology research. It is small in size and contains a multiple cloning site for inserting DNA fragments, as well as antibiotic resistance genes for selection in bacteria.
What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?
The enzyme produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18 is luciferase. This enzyme is responsible for the bioluminescent properties of animals like fireflies and glowworms. Cells transformed with plasmid lux will emit light in the presence of the substrate luciferin, whereas cells transformed with pUC18 will not.
Why do the cells transformed with pUC18 and plasmid lux grow in the presence of ampicillin?
The pUC18 plasmid contains the ampicillin resistance gene (ampR) which confers resistance to ampicillin. The Lux operon on the plasmid allows for bioluminescence production and acts as a reporter gene. Therefore, transformed cells that harbor both plasmids can grow in the presence of ampicillin due to pUC18 and express bioluminescence due to the Lux operon.
What would have happened if we had cut both the jellyfish glo gene and the puc18 plasmid with the EcoR1 restriction enzyme?
If both the jellyfish glo gene and the puc18 plasmid were cut with the EcoRI restriction enzyme, compatible sticky ends would be generated on both DNA fragments. This would allow the jellyfish glo gene to be inserted into the puc18 plasmid through a process called ligation. As a result, the plasmid could be used to clone the glo gene, facilitating its expression in a host organism for further study or application. This technique is a fundamental method in genetic engineering and molecular biology.
What is the abbreviation for an associate's degree?
The abbreviation is AAS.The abbreviation is AAS.The abbreviation is AAS.The abbreviation is AAS.The abbreviation is AAS.The abbreviation is AAS.
What ai abbreviation for abbreviation?
The abbreviation for abbreviation is "abbr."
Abbreviation for centimeter?
The abbreviation for centimeter is cm. The abbreviation for meter is m. The abbreviation for inches is in. The abbreviation for liter is L.
What is the abbreviation of GPA?
"GPA" is already an abbreviation for "grade point average". There is no further abbreviation for the abbreviation.
What is the abbreviation for parents?
Parent is actually an abbreviation itself. It is an abbreviation for parental. Some say that an abbreviation could be rent, but other than that since it is an abbreviation it doesn't really have an abbreviation.
What is Illinois' postal abbreviation?
Standard Abbreviation: Ill.Postal Abbreviation: IL
