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DNA ligase links together two DNA strands that have double-strand break (a break in both complementary strands of DNA). DNA ligase creates a phosphodiester bond to fully repair the DNA.

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Which enzyme do scientists use to bond a new gene to plasmid DNA?

Scientists use DNA ligase to bond a new gene to plasmid DNA. DNA ligase catalyzes the formation of phosphodiester bonds between the ends of the new gene and the plasmid, creating a recombinant DNA molecule.


Which enzyme do scientists use to bond a new gene to a plasmid?

DNA ligase


What enzyme joins pieces of DNA together?

Ligase is used to join DNA strands together


What enzyme pastes the sticky ends of a DNA?

It is called DNA ligase. Catalyzes the formation of a phosphodiester bond between a 3'-hydroxyl group and a 5'-phosphate group in DNA. This enzyme catalyzes the joining together of two single-stranded DNA segments which may be either parts of the same duplex or parts of different duplexes. This enzyme functions in DNA replication and in DNA repair by linking DNa fragments together.In biotechnology, is widely used the DNA ligase from bacteriophage T4 that catalyzes the formation of a phosphodiester bond between adjacent 3'-OH and 5'-P termini in DNA.


How does ligase work to join DNA fragments together?

Ligase is an enzyme that helps to join DNA fragments together by forming a strong bond between the sugar-phosphate backbone of the DNA strands. This process is essential for DNA replication, repair, and recombination.


What enzyme binds fragments of DNA on the lagging strand?

DNA ligase is the enzyme that binds together the Okazaki fragments on the lagging strand during DNA replication. It forms phosphodiester bonds between adjacent nucleotides to create a continuous strand of DNA.


The enzyme that can join pieces of DNA together is?

DNA-polymerase synthesizes DNA molecules, while DNA-ligase ca join DNA pieces together by making phosphodiester bond.


What is the mechanism of action of DNA ligase in a DNA ligase reaction?

DNA ligase is an enzyme that plays a crucial role in the process of DNA replication and repair by joining together the ends of DNA strands. It works by forming a phosphodiester bond between the phosphate group of one DNA strand and the hydroxyl group of another, effectively sealing the break in the DNA backbone. This process helps to ensure the integrity and stability of the DNA molecule.


What is the job of the DNA ligase?

It joins Okazaki fragments


What molecule catalyzes covalent bond formation between DNA?

DNA ligase catalyzes the formation of covalent bonds between nucleic acid strands, such as DNA. It acts by joining the sugar-phosphate backbone of two DNA fragments together to repair DNA damage or during DNA replication.


How does DNA ligase utilize ATP during the process of DNA repair and replication?

DNA ligase utilizes ATP during the process of DNA repair and replication by using the energy from ATP to catalyze the formation of a phosphodiester bond between the DNA strands, sealing any breaks or gaps in the DNA molecule.


The enzyme that restores the phosphodiester linkage between adjacent fragments in the lagging strand during DNA replication is?

DNA ligase. It plays a crucial role in DNA replication by creating a phosphodiester bond between the Okazaki fragments in the lagging strand to complete the DNA synthesis process.