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It is called DNA ligase. Catalyzes the formation of a phosphodiester bond between a 3'-hydroxyl group and a 5'-phosphate group in DNA. This enzyme catalyzes the joining together of two single-stranded DNA segments which may be either parts of the same duplex or parts of different duplexes. This enzyme functions in DNA replication and in DNA repair by linking DNa fragments together.
In biotechnology, is widely used the DNA ligase from bacteriophage T4 that catalyzes the formation of a phosphodiester bond between adjacent 3'-OH and 5'-P termini in DNA.
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What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
What are stick ends?
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
what substance was needed to join the insulin gene to the bacterial DNA?
Technically it is not a substance, but the DNA itself. Let me explain. When the insulin gene is cut out of a regular strand, it is done through a DNA ligase (a cutting enzyme). The SAME ligase is used to cut the bacterial loop of DNA. When ligase cuts DNA sticky ends are left. These sticky ends are, as they say, sticky, and will readily join to new bases with the corresponding (complementary) base pair sequence. As the same ligase is used, the corresponding base sequence is inside the bacterial DNA, so they should connect together.
Sticky ends are produced as a result of the action of?
restriction enzymes on DNA molecules.
What is the function of the enzyme DNA ligase?
DNA ligase is an enzyme that plays a crucial role in the process of DNA replication and repair by joining together the ends of DNA strands to form a continuous strand.
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
A restriction enzyme is likely to cut which kind of molecules?
DNA molecules. A strand of DNA molecules can be cut to have blunted ends or jagged ends (sticky ends).
What does the term sticky ends refer to in gene splicing?
Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.
Which enzyme should she use to join the sticky ends of the gene and the plasmid?
She should use a DNA ligase enzyme to join the sticky ends of the gene and the plasmid. DNA ligase catalyzes the formation of phosphodiester bonds between the nucleotides of the gene and the plasmid, sealing them together.
Which protein creates DNA fragments with sticky ends?
Restriction enzymes are proteins that can create DNA fragments with sticky ends by cleaving DNA at specific recognition sequences. The sticky ends refer to single-stranded overhangs that are complementary to each other, allowing for the fragments to easily anneal to each other during DNA recombination.
How can you convert sticky ends into blunt ends?
To convert sticky ends into blunt ends, you can use the enzyme DNA polymerase to fill in the overhanging nucleotides. By adding the appropriate nucleotides complementary to the sticky ends, you effectively create blunt ends. Alternatively, you can use exonucleases to trim back the sticky ends, removing the overhangs and resulting in blunt ends. Both methods allow for the subsequent ligation of DNA fragments.
Which enzyme is added to attach the DNA backbone between vetor and foreign DNA that have formed bases pairs at sticky ends during of recombinant DNA?
DNA ligase is added.
Which enzyme should be used to join the sticky ends of the gene and the plasmid?
To join the sticky ends of a gene and a plasmid, DNA ligase should be used. This enzyme facilitates the formation of phosphodiester bonds between the adjacent nucleotides, effectively sealing the nicks in the sugar-phosphate backbone of the DNA. It is commonly employed in molecular cloning to create recombinant DNA molecules.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
An enzyme called what is added to attach the DNA backbone between vector and foreign DNA that have formed base pairs at the sticky ends during the formation of recombinant dna?
D ligase
How can a restriction enzyme leave sticky ends between DNA fragments?
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
