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yes an organism can be MR & VP positive both. for example staphylococcus.

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M-R (Methly Red) is a test used primarily to identify gram-negative rods. M-R is a pH indicator that changes when the pH is below 4.5. The indicator will turn red below 4.5 and yellow, in a pH above that point.

V-P (Voges-Proskauer) is a specific test for metabolic intermediates when sugars ferment to produce butylene glycol. This does occur is some gram negative rods.

A positive V-P test is almost never observed with a positive M-R test. The M-R test is based on the production of sufficient acid to lower the pH to below 4.5. If some of the sugar being fermented is being used to make butylene glycol, then it cannot be used to also produce acids.

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Could an organism be a fermenter and also be both MR and V-P negative?

Yes, an organism can be a fermenter and be both MR (methyl red) and V-P (Voges-Proskauer) negative. Fermentation is a metabolic process that converts sugar into acids, alcohols, or gases, but the MR and VP tests specifically evaluate different aspects of sugar metabolism that may not be related to fermentation. So, an organism can still ferment sugars without giving positive results on these tests.


What does a positive Voges-Proskauer test mean?

It means that the bacterial organism uses the butylene glycol pathway; producing acetoin (acetylmethylcarbinol). This specific compound is produced only by bacteria that utilize this pathway. Usually (but not always) a positive Voges-Proskauer (VP) test indicates the organism is negative for the methyl red (MR) test. Both the MR and VP tests are usually done using the same culture; after dividing it into two equal volumes.


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Related Questions

Could an organism be both MR and V-P positive?

An organism can not test positive for both an MR test and a V - P test. The MR test is an abbreviation for Methyl Red and it tests for E coli The V - P is an abbreviation for Vogue Proskauer and it tests for enterobacter and klebsiella.


Could an organism be a fermenter and also be both MR and V-P negative?

Yes, an organism can be a fermenter and be both MR (methyl red) and V-P (Voges-Proskauer) negative. Fermentation is a metabolic process that converts sugar into acids, alcohols, or gases, but the MR and VP tests specifically evaluate different aspects of sugar metabolism that may not be related to fermentation. So, an organism can still ferment sugars without giving positive results on these tests.


Organism which is indole negative mr positive vp negative citrate positive?

Sounds like Citrobacter freundii. Double check that you do have a gram negative rod that is motile, H2S negative and oxidative negative (most gram neg rods are).


What does a positive Voges-Proskauer test mean?

It means that the bacterial organism uses the butylene glycol pathway; producing acetoin (acetylmethylcarbinol). This specific compound is produced only by bacteria that utilize this pathway. Usually (but not always) a positive Voges-Proskauer (VP) test indicates the organism is negative for the methyl red (MR) test. Both the MR and VP tests are usually done using the same culture; after dividing it into two equal volumes.


Organism which is citrate positive indole negative MR negative VP negative sorbitol negative?

The organism is likely to be Escherichia coli. E. coli is citrate positive since it can utilize citrate as a carbon source, but it is indole negative as it does not produce indole from tryptophan. Additionally, E. coli is MR (methyl red) negative and VP (Voges-Proskauer) negative, indicating its fermentation pattern. Lastly, E. coli is typically sorbitol negative, meaning it does not ferment sorbitol.


Aerobic Bacteria that is citrate positive VP negative and MR positive?

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Why is A positive VP test is almost never observed with a positive MR test?

its obvious!! methyl red test tells us if the bacteria is using mixed acid fermentation process. if it is following it will show +ve. vp test is used to detect whether acetotin is produced or not.if bacteria is using mixed acid fermentation process acetotin will not be produced. so our bacteria can be either mr +ve or vp -ve. or it can be both negative....it cant be both +ve for further doubts ping me at sandeepdsensation@gmail.com


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