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Why was Mycobacterium smegmatis used instead of Mycobacterium tuberculosis?

Mycobacterium smegmatis is a non pathogenic organism so it is used in labs to study about Mycobacterium tuberculosis .


What color is mycobacterium smegmatis?

Mycobacterium smegmatis is a nonpigmented bacterium, meaning that it does not produce any distinctive color on its own. It appears as colorless or pale under the microscope.


What diseases are caused by mycobacterium smegmatis?

Mycobacterium smegmatis is not a major human pathogen and is considered non-pathogenic to humans. It is commonly used as a model organism in research due to its genetic similarity to other pathogenic mycobacteria.


What shape does mycobacterium smegmatis have?

Mycobacterium Smegmatis is a bacilli bacteria. It is rod shaped.


Is Mycobacterium Smegmatis a spore former?

M.smegmatis is strictly a non-spore former. It neither forms spores nor capsules.


M smegmatis acid fast?

Mycobacterium smegmatis is a fast-growing species of mycobacteria, but it is not acid-fast like the pathogenic mycobacteria such as Mycobacterium tuberculosis. Acid-fast staining is a characteristic feature of mycobacteria that have a waxy lipid layer in their cell wall, which makes them resistant to staining by conventional methods.


Does mycobacterium smegmatis have flagella?

All Mycobacteria are non-flagellated. Originally thought to be non-motile, it has recently been discovered M. smegmatis and other species of Mycobacteria move using a sliding mechanism in which the organism flattens and retracts to move.


How would an endospore stain of mycobacterium appear?

Acid fast Mycobacterium have a waxy molecule in their cell wall that will take up and retain the malachite green stain when subjected to the endospore staining process. The uniformly green appearance of endospore stained acid fast cells doesn't mean they produce endospores. These are vegetative cells that have taken up color from the heat driving malachite green into their waxy cell wall.


Are endospores acid fast?

Yes, endospores are acid-fast because their thick walls are resistant to most stains and dyes, including the acid-fast stain used in microbiology to detect mycobacteria like Mycobacterium tuberculosis. This resistance allows endospores to retain the stain even after decolorization with acid-alcohol.


Why do we target IS 6110 for the identification of Mycobacterium tuberculosis?

The reason we use IS6110 for the identification of TB using procedures such as RFLP and Spoligotyping for several years now is because this is a genetic insertion sequence (IS) or element that is found exclusively within the members of the Mycobacterium tuberculosis complex (MTBC). Some researchers have found IS6110-like elements in some other mycobacteria such as M. smegmatis but because smegmatis do not cause TB, its clinical importance is lesser. This info was taken from "IS6110 is found in non-M. tuberculosis complex species" by Coros A, DeConno E, and Derbyshire KM, from the Wadsworth Center, NY, NY, (AMS March 7, 2008)


Is the mycobacterium smegmatis positive to acid-fast?

Yes, Mycobacterium smegmatis is positive for acid-fast staining due to its thick, waxy cell wall. This characteristic allows it to resist decolorization by acid-alcohol during the staining process, leading to retention of the primary stain (carbol fuchsin) and appearing pink or red under a microscope.


Does a saprophyte have endospores?

Endospores are able to exist for hundreds of years before they resume growth. Saprophytes are decomposing bacteria that contain endospores.