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The spectrophotometer measures total cell count, including both live and dead cells.

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What is total viable count of bacteria?

Total viable count is a method used to estimate the total number of viable bacteria in a sample. This is typically done by plating a diluted sample onto an agar plate and counting the number of colonies that grow. It provides an estimate of the total number of bacteria that are able to grow and reproduce under the specific conditions used in the assay.


What is total plate count in water?

Total Plate Count (TPC) in water is a measure of the total number of viable bacteria in a given sample of water. It is used as an indicator of overall water quality and can help determine if the water is safe for consumption or other purposes. High TPC levels may indicate contamination and potential health risks.


What is affect of spores on tpc count?

Spores do not contribute to the total plate count (TPC) as they do not metabolize on standard culture media used for TPC determination. Only viable bacteria that can grow and form colonies will affect the TPC count. Spores may appear on plates as the result of sporulation by certain bacteria, but they are not included in the TPC calculation.


Why should the concentration of sample be low in spectrophotometer readings?

When doing reading on a spectrophotometer, the sample being studied is either a color change or a precipitated compound, depending on the wavelength that it is being read. If it is a precipitated compound and it has a very high concentration, then you run the risk of the light being used to measure the absorbance not going through. In which case you have total absorbance but it is inaccurate in helping you determine the concentration of your sample because you are unsure where the concentration limit is for that wavelength, and your sample could possibly be able to absorb more. In which case you still can't calculate the concentration of the sample.


Does a colony count really give a total count of microorganisms per unit sample of the original material?

Yes, each colony that forms on the plate was the result of a single microorganism. If you can know the quantity of the unit sample, you can know the number of microorganisms that were in that sample by counting the colonies.

Related Questions

Name the units used for measuring micro-organisms.?

Total Viable Count,(TVC) or Total bacterial Count,(TBC)


What is total viable count of bacteria?

Total viable count is a method used to estimate the total number of viable bacteria in a sample. This is typically done by plating a diluted sample onto an agar plate and counting the number of colonies that grow. It provides an estimate of the total number of bacteria that are able to grow and reproduce under the specific conditions used in the assay.


Does the number of bacteria recorded by the standard plate count method accurately reflect the total bacterial count from the sample?

No. The standard plate count method is an indirect measurement of cell density of only viable bacterial cells. Optical density counting measure entire bacterial sample, the living as well as the dead bacterial cells.


What does TVC stand for in meat?

TVC stands for Total Viable Count and is usually referred to when counting bacteria in meat.


What is total plate count in water?

Total Plate Count (TPC) in water is a measure of the total number of viable bacteria in a given sample of water. It is used as an indicator of overall water quality and can help determine if the water is safe for consumption or other purposes. High TPC levels may indicate contamination and potential health risks.


Why direct microscopy and plate count give not a same counts?

Direct microscopy counts viable and non-viable cells, whereas plate count only counts viable cells that are able to grow and form colonies on agar plates. Additionally, plate count may underestimate the total number of viable cells due to factors like the inability of certain cell types to grow under specific conditions or the formation of aggregated cells that do not separate easily on the agar plate.


How can you determine the least count of spherometer?

To determine the least count of a spherometer, you measure the total number of divisions on the circular scale and divide it by the total travel range of the micrometer screw. The least count is the smallest incremental distance that can be measured accurately by the spherometer.


When economists use dollars to count the total value of goods and services they are using money as a?

Measure Of Value


What is affect of spores on tpc count?

Spores do not contribute to the total plate count (TPC) as they do not metabolize on standard culture media used for TPC determination. Only viable bacteria that can grow and form colonies will affect the TPC count. Spores may appear on plates as the result of sporulation by certain bacteria, but they are not included in the TPC calculation.


Does the work cited count towards the total word count?

No, the work cited does not count towards the total word count.


How do you get a total from a group of averages?

Average = Total/Count so Total = Average*Count.


How can I count chain links accurately?

To count chain links accurately, lay the chain flat and use a ruler to measure the length of a certain number of links. Divide the total length by the length of one link to determine the total number of links in the chain.