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How Pulsed-Field Gel Electrophoresis differences from PCR identification of bacterial strain?
In using PCR to identify a bacterial strain, a single segment of DNA is amplified, typically an rRNA gene or a portion of it. The amplified fragment is then sequenced and the sequence compared to that of sequences in the databases. Since rRNA gene sequences are so conserved, this method does not have the resolution to distinguish individual strains of the same species, although it can be used for species identification.
In PFGE, there is no amplification. Instead, restriction enzymes are used to digest chromosomal DNA to generate a characteristic pattern. Also, the detection method is staining of the DNA, not DNA sequencing
What else can I help you with?
Can the API20E kit used for the identification of any bacterial strain?
The API 20E kit is designed primarily for the identification of Enterobacteriaceae and some other gram-negative bacteria commonly found in clinical specimens. While it may be useful for the identification of certain bacterial strains, it is not comprehensive and may not cover all strains. Additional testing may be needed for accurate identification.
What is the definitive published source for bacterial classification?
The definitive published source for bacterial classification is Bergey's Manual of Systematic Bacteriology. It provides comprehensive information on the classification, identification, and nomenclature of bacteria.
What strategies would allow you to determine if a bacterial colony had been infected with a lysogenic virus?
One strategy would be to test the bacteriophage replication cycle, looking for signs of lysogenic cycle genes or prophage induction. Additionally, genomic sequencing can help identify viral genetic material integrated into the bacterial genome, indicative of lysogeny. Lastly, observing any phenotypic changes in the bacterial colony, like increased resistance or altered growth patterns, may suggest lysogenic infection.
What is the major differences between a bacterial cell and the cell of other organisms?
All bacterial cells do not have a nucleus but the cells of other animal have nucleus which is usually spherical or oval in shape inside it there are chromosomes that form a dense tangle referred to as chromatin.
How can turbidity be used to count bacteria?
Turbidity can be used to estimate bacterial concentration in a sample by measuring the cloudiness or haziness caused by suspended particles, including bacteria. A higher turbidity level indicates a greater number of suspended particles, which can correlate with higher bacterial counts. While turbidity provides a rapid and indirect measure of bacterial presence, it may not differentiate between types of bacteria or account for non-bacterial particles, necessitating further analysis for accurate identification.
What are the differences between agar and agarose, and how do they impact their respective uses in laboratory applications?
Agar is a polysaccharide derived from seaweed, while agarose is a purified form of agar. Agar is used for bacterial and fungal cultures, while agarose is used for electrophoresis to separate DNA and proteins based on size. The differences in composition and purity impact their effectiveness in specific laboratory applications.
Antibiotics rely upon differences in the structure of human and bacterial?
ribosomes
What has the author R A Lelliott written?
R. A. Lelliott has written: 'Methods for the diagnosis of bacterial diseases of plants' -- subject(s): Bacterial diseases of plants, Diagnosis, Identification, Phytopathogenic bacteria
Can the API20E kit used for the identification of any bacterial strain?
The API 20E kit is designed primarily for the identification of Enterobacteriaceae and some other gram-negative bacteria commonly found in clinical specimens. While it may be useful for the identification of certain bacterial strains, it is not comprehensive and may not cover all strains. Additional testing may be needed for accurate identification.
What is the definitive published source for bacterial classification?
The definitive published source for bacterial classification is Bergey's Manual of Systematic Bacteriology. It provides comprehensive information on the classification, identification, and nomenclature of bacteria.
What is the differences between viral and bacterial water-born disease?
The viral water-borne diseases cannot be treated by the antibiotics while the bacterial water-borne diseases can be treated by the antibiotics.
What is the purpose of bacterial staining?
Bacterial staining is used to visualize and differentiate bacteria based on their cell wall composition, shape, and arrangement. This technique helps in identification and classification of bacteria, as well as in distinguishing between different types of bacteria in clinical diagnoses and research. Additionally, bacterial staining is useful for studying bacterial morphology, structure, and cellular processes.
What are the differences in symptoms of viral meningitis and bacterial meningitis?
There is really no difference between the symptoms of viral and bacterial meningitis. With both you will have fever, headache, stiff neck, vomiting, and sensitivity to light. However that being said bacterial comes on suddenly where viral can take a few days.
Why is gel electrophoresis important?
To separate and analyze DNA fragments and protein fragments by weight. If you have digested some bacterial DNA, for instance, then you can tell by running the fragmented DNA in the gel whether you have digested the correct base length.
Why characterization and identification of bacteria?
Characterization and identification of bacteria is important for several reasons. Firstly, it helps in understanding the taxonomy and diversity of bacterial species, which is crucial for studying their ecological roles and evolutionary relationships. Secondly, it allows for the diagnosis and treatment of bacterial infections, as different species may require different antimicrobial therapies. Lastly, it aids in tracking the spread of bacterial pathogens and monitoring their antibiotic resistance patterns, helping to guide public health interventions and prevent outbreaks.
What is the meaning of bateriology?
Bacteriology is the branch of science dedicated to the study of bacteria. It is a subdivision of microbiology that involves the identification and classification of bacterial species.
Why is surgery recommended for a CNS infection?
Surgery allows immediate relief of pressure on the brain or spinal cord, as well as an opportunity to collect infectious material for bacterial identification.
