Discrete colonies on an agar gel plate can be identified by their clear separation from one another, with a defined boundary around each colony. They typically appear as individual, well-isolated clusters of cells, often showing distinct shapes, sizes, and colors. Additionally, the lack of merging or overlapping between colonies indicates that they are discrete and not competing for resources. Observing these characteristics helps in distinguishing individual microbial species in mixed cultures.
That really depends on what agar you are growing them on. If you are using growing agars such as BHI or blood, both will grow, and you won't be able to tell the difference when looking at them on the plate. If you use a selective agar that, lets say, will only grow cocci, then the bacilli won't grow. Or vice versa, if your selective agar only grows bacilli, then the cocci won't grow. If you use a differential agar, it may turn one a different color than the other -- for example, it may turn the bacillus blue, and the cocci may turn red. It is all dependent on the media you choose to grow your samples.
Since you did not tell us WHICH plate "this" one is, we cannot answer correctly
Nothing, it's a theory, like evolution!
P. aeruginosa is not alpha hemolytic. Hemolysis is used mainly for the identification of Streptococci and can be used for Staphylococci. P. aeruginosa can be mistaken for being alpha hemolytic because it produces a green diffusable pigment. This pigment is especially visible if grown in media with little to no pH dye indicators such as SIM tubes, nutrient broths (undisturbed), API strips, etc. A good way to tell the difference between diffusable pigment production and hemolysis is to look at the agar surrounding isolated colonies. Diffusable pigments should be alot more visible in areas with higher concentrations of bacteria (quadrant 1) and may not be seen around isolated colonies of 24 hour cultures. Hemolysis should be readily seen around isolated colonies regardless of incubation time. If left long enough, P. aeruginosa will eventually clear all the RBC in the agar around the colonies (beta hemolysis).
During telophase of mitosis, a cell plate is formed as the plant cell begins its division. In animal cells, the cell pinches in the center to form two cells; no cell plate is laid down.
Yes, agar agar can expire. To determine if it is still safe to use, check the expiration date on the packaging. Additionally, inspect the agar agar for any signs of discoloration, unusual odor, or mold growth. If any of these are present, it is best to discard the agar agar to avoid any potential health risks.
That really depends on what agar you are growing them on. If you are using growing agars such as BHI or blood, both will grow, and you won't be able to tell the difference when looking at them on the plate. If you use a selective agar that, lets say, will only grow cocci, then the bacilli won't grow. Or vice versa, if your selective agar only grows bacilli, then the cocci won't grow. If you use a differential agar, it may turn one a different color than the other -- for example, it may turn the bacillus blue, and the cocci may turn red. It is all dependent on the media you choose to grow your samples.
when you plate E.coli onto a EMB plate the colonies turn shinie metalic green, EMB is selective for gut bacteria and it diferentiates E.coli.
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
"I hope you do not tell them, I want this to be discrete."==The sentence, "I hope you do not tell them, I want this to be discrete" illustrates the meaning of discreet, a word that is often confused with discrete("having or showing discernment or good judgment in conduct and especially in speech : PRUDENT; especially, capable of preserving prudent silence" -- quoted from Merriam-Webster Collegiate online)discrete means "constituting a separate entity: individually distinct"A sentence that illustrates that meaning is, "The plan contains several discrete sections."
No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured, think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.
You will see different color colonies, irregular ones, or round and even ones on a streak plate.
First, you need to tell us WHICH colonies. In which country.
The graph of a continuous function will not have any 'breaks' or 'gaps' in it. You can draw it without lifting your pencil or pen. The graph of a discrete function will just be a set of lines.
Well, Macconkey Agar II plates have Gram Crystal Violet in them so there are a number of bacterial species that can absorb it and turn "very pink." The way to narrow it down is to identify whether it grows in chains or clusters. But other than that, it is hard to tell.
if it has a linceses plate
Bacteria growth can be detected by an increase in turbidity (cloudiness) of the culture, formation of colonies on agar plates, or by changes in pH or color of the medium due to metabolic byproducts. Additionally, observing the presence of a pellicle, sediment, or turbidity in a liquid culture can indicate bacterial growth.