The last step in transcription is termination, where the RNA polymerase enzyme stops adding nucleotides to the growing mRNA strand and detaches from the DNA template. This results in the release of the newly formed mRNA molecule.
mRNA
The maximum length of a polypeptide encoded by an mRNA is determined by the number of nucleotides in the mRNA sequence. Each amino acid is encoded by a set of three nucleotides called a codon. With a 45-nucleotide mRNA sequence, the maximum length of the polypeptide would be 45/3 = 15 amino acids.
According to "Elementary Biochemistry" by J. Davies and B. Shaffer Littlewood, mRNA contains 100-5000 N-containing bases, which is also the # of nucleotides. I guess the size of the mRNA is related to the size of the protein molecule that is being synthesized. What is the largest protein made in the human body?
The creation of mRNA is called transcription. mRNA is being transcribed from the DNA template.
The presence of a 5' cap and a poly-A tail at the ends of the mRNA molecule help protect it from degradation by exonucleases. Additionally, secondary structures within the mRNA and specific sequences can affect stability and longevity.
The last step in transcription is termination, where the RNA polymerase enzyme stops adding nucleotides to the growing mRNA strand and detaches from the DNA template. This results in the release of the newly formed mRNA molecule.
mRNA
The maximum length of a polypeptide encoded by an mRNA is determined by the number of nucleotides in the mRNA sequence. Each amino acid is encoded by a set of three nucleotides called a codon. With a 45-nucleotide mRNA sequence, the maximum length of the polypeptide would be 45/3 = 15 amino acids.
The poly(A) tail is a long sequence of adenine nucleotides added to the end of a processed mRNA molecule. It plays a role in protecting the mRNA from degradation and regulating its stability and translation efficiency. The addition of the poly(A) tail is an essential step in mRNA processing in eukaryotic cells.
A codon is exactly three bases long, so an mRNA strand with 60 bases would contain 20 codons. The first codon will encode for methionine (this is called the "start" codon) and the last codon will be a "stop" codon, which does not encode for an amino acid. Thus, an mRNA strand of 60 bases will code for 19 amino acids. Keep in mind, it is possible for a stop codon to be anywhere on the mRNA strand, and when a stop codon reaches the ribosome, translation must stop. For example, if an mRNA strand contained 30 codons, and the 15th were a stop codon, the mRNA would only code for 14 amino acids and then be done. The other 15 codons would go untranslated.
According to "Elementary Biochemistry" by J. Davies and B. Shaffer Littlewood, mRNA contains 100-5000 N-containing bases, which is also the # of nucleotides. I guess the size of the mRNA is related to the size of the protein molecule that is being synthesized. What is the largest protein made in the human body?
The cap and tail on eukaryotic mRNA play important roles in mRNA stability and translation. The 5' cap protects the mRNA from degradation and helps in the initiation of translation. The poly(A) tail at the 3' end of mRNA also plays a role in mRNA stability and regulation of translation.
Yes, cDNA is complementary to mRNA.
The creation of mRNA is called transcription. mRNA is being transcribed from the DNA template.
DNA -> transcription -> pre-mRNA -> mRNA processing -> mRNA -> translation -> protein
No, transcription involves the formation of mRNA.