S. mitis stains purple and therefore is a gram positive bacteria
S. arureus is a gram posotive cocciScientific classificationDomain:BacteriaKingdom:EubacteriaPhylum:FirmicutesClass:BacilliOrder:BacillalesFamily:StaphylococcaceaeGenus:StaphylococcusSpecies:S. aureus
The process of gram staining is simple. 1)smear bacteria from pure culture onto slide, heat fix 2)flood with crystal violet (1min) 3)Add iodine (1 min) 4)acid/alcohol wash (1 min) 5)Flood with safranine (1min) 6)Air dry and examine. These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close). Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer. Source(s): Medical Microbiology
In terms of microbiology, S. aureus and S. epi can be differentiated through a number of metabolic tests. The tests being Methyl Red, Vogues-Prauskauer, Mannitol fermentation (of which S. aureus would be + and S. epi - ) and Oxidase (S. aureus - and S. epi + ).
Staphylococcus epidermidis is a Gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure. This results in a purple color under the microscope.
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
S. epidermidis is a Gram-positive bacterium. It appears purple when subjected to a Gram stain due to its thick peptidoglycan layer in the cell wall.
S. arureus is a gram posotive cocciScientific classificationDomain:BacteriaKingdom:EubacteriaPhylum:FirmicutesClass:BacilliOrder:BacillalesFamily:StaphylococcaceaeGenus:StaphylococcusSpecies:S. aureus
Frimbriae are structures related to Gram-negative bacteria. S. Pneumoniae, however, is Gram-positive. Therefore it does not have fimbriae.
A 7-day-old culture may appear gram negative, when the organmisms are actually gram positive. Thus, S. epi would appear gram negative, and E. coli gram negative.
Post ID Special:Contributionssaid: "blue-violet.....because it is a gram-negative bacteria"Wrong...the color is correct, but S. aureus is gram-POSITIVE.
The process of gram staining is simple. 1)smear bacteria from pure culture onto slide, heat fix 2)flood with crystal violet (1min) 3)Add iodine (1 min) 4)acid/alcohol wash (1 min) 5)Flood with safranine (1min) 6)Air dry and examine. These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close). Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer. Source(s): Medical Microbiology
In terms of microbiology, S. aureus and S. epi can be differentiated through a number of metabolic tests. The tests being Methyl Red, Vogues-Prauskauer, Mannitol fermentation (of which S. aureus would be + and S. epi - ) and Oxidase (S. aureus - and S. epi + ).
The rod shape(bacilus) and the sphiral(sphirily) have flagella. They both have same structure of flagela but there is a difference between gram positive and gram negative,s flagellum structure.
G is always negative when H is negative and S is positive.
Staphylococcus epidermidis is a Gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure. This results in a purple color under the microscope.
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
Acid fast organism would be gram positive.----------------------------------------------------------------------------------------------------The above answer is incorrect. Acid-fast bacteria would be "gram negative."Bacteria such as these have very different cell walls which contain a great deal of waxy material, although they still contain peptidoglycan. This waxy material prevents stain penetration, rendering the cell invisible.Since these acid-fast bacteria are not stained by Gram's Stain Method they have been characterized as "gram negative."----------------------------------------------------------------------------------------------------Heres what the text book says. This is a tricky question based on opinion."Their cell walls are unique among the gram-positive bacteria because they consist of other layers in addition to peptidoglycan. An important component of their walls is a group of fatty acids called mycolic acids. Some have proposed that the mycolic acids form a bilayer structure that is analogous to the outer membrane of gram-negative bacteria."So answer one is technically correct but they characteristics as those of gram negative cells (answer 2).Page 58Prescott's MicrobiologyEdition: 8thAuthor(s):Willey; Sherwood; WoolvertonISBN13:9780073375267ISBN10:0073375268Format:HardcoverPub. Date:1/1/2010Publisher(s):MCGRAW HILL