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The proton gradient caused by the high concentration of protons in the intermembrane space begins another process called chemiosmosis. The protons "want" to diffuse back into the mitochondrial matrix and to a lower concentration. A very special enzyme in the membrane called ATP synthase will take protons and transport them into the matrix. In doing so, energy is released and used to phosphorylate ADP into ATP.
This process of producing ATP differs from substrate-level phosphorylation. See related links and questions below for more information.
What else can I help you with?
What type of phosphorylation does not require a membrane?
Cytoplasmic or soluble enzymes can carry out phosphorylation that does not require a membrane. This type of phosphorylation occurs in the cytoplasm or within organelles like the mitochondria and does not involve a membrane-bound protein complex.
Will phosphorylation denature a protein?
Phosphorylation typically does not denature a protein. Phosphorylation is a reversible modification where a phosphate group is added to a protein, often regulating its function, structure, or localization within the cell. However, extreme or incorrect phosphorylation can lead to protein misfolding and dysfunction.
What are two methods of phosphorylation?
Two methods of phosphorylation are: Enzyme-catalyzed phosphorylation, where enzymes like kinases transfer phosphate groups from ATP to specific proteins. Photo-phosphorylation, which occurs during photosynthesis where light energy is used to convert ADP and inorganic phosphate into ATP.
What does phosphorylation do to a moleclue?
it adds a phosphate group Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule.
What are the different types of phosphorylation and where do they occur?
Phosphorylation primarily occurs in two forms: substrate-level phosphorylation and oxidative phosphorylation. Substrate-level phosphorylation occurs in the cytoplasm during glycolysis and in the mitochondria during the citric acid cycle, where ATP is produced directly from a substrate. Oxidative phosphorylation takes place in the inner mitochondrial membrane, involving the electron transport chain and chemiosmosis, where ATP is generated indirectly through the flow of protons across the membrane. Additionally, there is protein phosphorylation, which is a regulatory mechanism occurring in various cellular locations, including the cytoplasm and nucleus, where proteins are modified by the addition of phosphate groups, often impacting their activity and function.
What type of phosphorylation does not require a membrane?
Cytoplasmic or soluble enzymes can carry out phosphorylation that does not require a membrane. This type of phosphorylation occurs in the cytoplasm or within organelles like the mitochondria and does not involve a membrane-bound protein complex.
What is the attachment of a phosphate group to a molecule called?
The attachment of a phosphate group to a molecule is called phosphorylation. This process often occurs in cellular signaling pathways and can change the activity or function of the molecule being modified.
Will phosphorylation denature a protein?
Phosphorylation typically does not denature a protein. Phosphorylation is a reversible modification where a phosphate group is added to a protein, often regulating its function, structure, or localization within the cell. However, extreme or incorrect phosphorylation can lead to protein misfolding and dysfunction.
What are two methods of phosphorylation?
Two methods of phosphorylation are: Enzyme-catalyzed phosphorylation, where enzymes like kinases transfer phosphate groups from ATP to specific proteins. Photo-phosphorylation, which occurs during photosynthesis where light energy is used to convert ADP and inorganic phosphate into ATP.
In fermentation is ATP produced by substrate level phosphorylation or oxidative phosphorylation or both or neither?
ATP in fermentation is typically produced by substrate-level phosphorylation, which involves the direct transfer of a phosphate group to ADP from a phosphorylated substrate. Oxidative phosphorylation, which involves the use of an electron transport chain to produce ATP, is not generally involved in fermentation.
What does phosphorylation do to a moleclue?
it adds a phosphate group Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule.
What are the different types of phosphorylation and where do they occur?
Phosphorylation primarily occurs in two forms: substrate-level phosphorylation and oxidative phosphorylation. Substrate-level phosphorylation occurs in the cytoplasm during glycolysis and in the mitochondria during the citric acid cycle, where ATP is produced directly from a substrate. Oxidative phosphorylation takes place in the inner mitochondrial membrane, involving the electron transport chain and chemiosmosis, where ATP is generated indirectly through the flow of protons across the membrane. Additionally, there is protein phosphorylation, which is a regulatory mechanism occurring in various cellular locations, including the cytoplasm and nucleus, where proteins are modified by the addition of phosphate groups, often impacting their activity and function.
What is the opposite of oxidative phosphorylation?
The opposite of oxidative phosphorylation is not a specific biological process, as it refers to the metabolic pathway that occurs in mitochondria to generate ATP from ADP using oxygen. However, an anaerobic process like fermentation can be considered as an alternative to oxidative phosphorylation.
Substrate level phosphorylation accounts for approximately what percentage of ATP formed during glycolysis?
100%. Substrate level phosphorylation accounts for about 10% of ATP generated by respiration. The other 90% is generated by oxidative phosphorylation.
Different between oxidative phosphorylation and substrate level phosphorylation?
Substrate-level phosphorylation occurs during Glycolysis and the Kreb's Cycle and involves the physical addition of a free phosphate to ADP to form ATP. Oxidative phosphorylation, on the other hand, takes place along the electron transport chain, where ATP is synthesized indirectly from the creation of a proton gradient and the movement of these protons back accross the membrane through the protein channel, ATP synthase. As the protons pass through, ATP is created.
Substrate-level phosphorylation can best be describe as?
Substrate-level phosphorylation can best be describe as the direct transfer of phosphate from one substrate to another. Oxidative phosphorylation is different from substrate level phosphorylation is that it generates ATP by using a proton motive force.
Another name for electron transport chain is?
The electron transport chain is also known as the respiratory chain.
