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What are the enzymes that are responisble for adding nucleotides to the exposed DNA bases during replication?
The enzymes responsible for adding nucleotides to the exposed DNA bases during replication are DNA polymerases. These enzymes catalyze the formation of phosphodiester bonds between adjacent nucleotides in the growing DNA strand based on the complementary base-pairing rule. Multiple types of DNA polymerases work together during DNA replication to ensure accurate and efficient synthesis of the new DNA strands.
What is enzyme that adds nucleotides to exposed nitrogen bases?
DNA polymerase is the enzyme that adds complementary nucleotides to exposed nitrogen bases during DNA replication.
Principle enzyme involved in DNA replication?
The primary enzyme involved in DNA replication is DNA polymerase. This enzyme is responsible for adding nucleotides to the growing DNA strand, which ensures accurate copying of the genetic information. There are different types of DNA polymerases with specific functions in the replication process.
Where D-loop replication is done?
D-loop replication occurs during the process of DNA repair in the mitochondria. This type of replication involves the formation of a displacement loop (D-loop) structure, which allows for the repair of damaged mitochondrial DNA by DNA polymerases.
Which mechanism(s) operate(s) to ensure the accuracy of DNA replication?
DNA replication accuracy is ensured primarily through three mechanisms: base pairing fidelity, proofreading by DNA polymerases, and mismatch repair. During replication, DNA polymerases incorporate nucleotides based on complementary base pairing, minimizing errors. Additionally, many DNA polymerases possess a proofreading ability, allowing them to remove incorrectly paired nucleotides immediately after they are added. Finally, if any mismatches persist, the mismatch repair system detects and corrects these errors post-replication, maintaining genomic integrity.
What are the enzymes that are responisble for adding nucleotides to the exposed DNA bases during replication?
The enzymes responsible for adding nucleotides to the exposed DNA bases during replication are DNA polymerases. These enzymes catalyze the formation of phosphodiester bonds between adjacent nucleotides in the growing DNA strand based on the complementary base-pairing rule. Multiple types of DNA polymerases work together during DNA replication to ensure accurate and efficient synthesis of the new DNA strands.
Enzymes that move along each of the DNA strands during replication adding nucleotides to the exposed bases?
DNA polymerases
What is the job of the DNA polymerases?
DNA polymerase adds bases to the 3' end during replication. It matches the c with G and A with U during replication. Never add to the 5' end!
What is enzyme that adds nucleotides to exposed nitrogen bases?
DNA polymerase is the enzyme that adds complementary nucleotides to exposed nitrogen bases during DNA replication.
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases, such as DNA polymerase III in prokaryotes and DNA polymerase delta in eukaryotes, have proofreading activities during DNA replication. These enzymes possess exonuclease activity, allowing them to detect and correct errors in newly synthesized DNA strands by removing misincorporated nucleotides. This proofreading function helps maintain the fidelity of DNA replication.
What does DNA polymerases use as their substrate?
The main substrate for for Taq polymerase is magnesium chloride
What enzyme reads the DNA template?
Various DNA polymerases read the DNA template during replication of DNA. Various RNA polymerases read the DNA template during transcription.
What are the two major functions that DNA polymerase performs?
DNA polymerase functions to catalyze the synthesis of new DNA strands by adding nucleotides to a growing DNA chain during replication. It also can recognize and correct errors in the newly synthesized DNA through its proofreading activity.
Compare the number of replication forks in prokaryotic and eukaryotic DNA during replication?
Prokaryotic DNA replication has a single origin of replication, leading to two replication forks. In contrast, eukaryotic DNA replication has multiple origins of replication, resulting in multiple replication forks forming along the DNA molecule.
Principle enzyme involved in DNA replication?
The primary enzyme involved in DNA replication is DNA polymerase. This enzyme is responsible for adding nucleotides to the growing DNA strand, which ensures accurate copying of the genetic information. There are different types of DNA polymerases with specific functions in the replication process.
Where D-loop replication is done?
D-loop replication occurs during the process of DNA repair in the mitochondria. This type of replication involves the formation of a displacement loop (D-loop) structure, which allows for the repair of damaged mitochondrial DNA by DNA polymerases.
What pairs with adenine during DNA replication?
DNA polymerases add nucleotides to the exposed base pairs according to base-pairing rules.
