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These are microrganisms that need some or little oxygen to grow, however, if they receive oxygen concentrations reach those found in air (20%), they are unable to grow. Hence they need the right amount of oxygen. The source http://mansfield.osu.edu/~sabedon/biol2020.htm

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Account for the subsurface growth of microaerophiles in a shake-tube culture?

Because microaerophiles require Oxygen, but at a low concentration.


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Using a semisolid nitrate medium with a small amount of agar allows microaerophiles, which require reduced oxygen levels, to grow without being exposed to high oxygen concentrations due to the agar's ability to create an oxygen gradient. This setup mimics the low-oxygen environments that microaerophiles thrive in and aids in their growth and identification.


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What kind of bacterium cannot tolerate oxygen?

The bacterium that cannot tolerate oxygen is Anaerobes, but there are different kinds and each has different tolerances when it comes to oxygen. Obligate Anaerobes can get killed by oxygen. Facultative anaerobes those can grow without oxygen. Aero-tolerant anaerobes they never use oxygen BUT they can tolerate it. Microaerophiles require i tiny bit of oxygen but anymore than the little bit they need and they cannot grow.


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Anthrax is a bacteria or virus?

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