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Although DNA can be isolated from a variety of sources, there are some general considerations for choosing a cell. They are:
- The cell should have a nucleus containing DNA
- The cell should be viable (alive). Although DNA can be isolated form dead cells as well, they are sometimes fragmented in dead cells therefore a viable cell is preferable for good quality DNA
- The cell should not be infected with a virus. If an infected cell is used, the viral genetic material will conaminate the source DNA
What else can I help you with?
What cells would I use to extract DNA from a living person?
Any cells can be used as you will find DNA in almost all of them (a few exceptions are red blood cells, platelets, etc.) but even where one specific cell in a tissue has no DNA many adjacent cells in the sample will have DNA.
How does the DNA work in cell cycles?
During interphase, the cell grows and makes a copy of its DNA. During the mitotic (M) phase, the cell separates its DNA into two sets and divides its cytoplasm, forming two new cells.
Why does a virus not have both DNA and RNA?
Because it doesn't use it's DNA or RNA to function, it uses it to inject into a cell and switch the cell's instructions to its own so the cell will make more viruses. Cells have DNA and RNA to tell the cell what to do, but viruses just do it naturally. Viruses have no use for both.
Can you see any internal structure in a bacteria?
You would have to use a Microscope, but yes.
Does DNA have mitochondria?
No, DNA does not have mitochondria. Mitochondria are structures found within cells that have their own DNA, separate from the DNA found in the cell's nucleus. Mitochondria are responsible for producing energy for the cell.
What part of the cell must be broken down to extract DNA?
In a eukaryote cell the double walled nuclear membrane must be broken, or pierced to release the DNA into the cytoplasm and then the phospholipid bilayer would have to be breached next to release the DNA from this type cell A prokaryote cell would need both its cell wall and its plasma membrane breached to release the DNA from the cell.
Explain how biologists get DNA out of a cell?
Biologists can extract DNA from a cell by first breaking open the cell to release its contents. They then use various techniques to separate the DNA from other cellular components, such as proteins and membranes. Common methods include using enzymes to break down proteins and alcohol precipitation to isolate the DNA.
Why do we use ethanol to extract DNA?
Ethanol is used to extract DNA because it is able to precipitate the DNA out of solution due to its nonpolar nature. When added to a DNA solution containing salt, the DNA molecules become less soluble in ethanol and can be easily collected by precipitation with a centrifuge.
Why do you have to use alcohol to extract DNA?
Because it is less dense than the alcohol and make it float
Why do scientists use the white blood cell when extracting DNA in gentic screening?
White blood cells (unlike red blood cells) have nuclei containing genetic material. Since DNA is present within the white blood cells and since blood is easy to obtain from the body, scientists use white blood cells to extract DNA
Can you use almond extract instead of vanilla extract?
You could use vanilla extract, but the taste would be different. you are bettere off going with almond instead
What cells would I use to extract DNA from a living person?
Any cells can be used as you will find DNA in almost all of them (a few exceptions are red blood cells, platelets, etc.) but even where one specific cell in a tissue has no DNA many adjacent cells in the sample will have DNA.
Some organisms use singlestranded rather than doublestranded DNA In these organisms which enzyme would probably not be needed for DNA replication?
Some viruses have single stranded DNA molecules. These viruses do not have the machinery to synthesise the DNA on their own. They insert their DNA in a living cell where the DNA synthesis takes place.
How does cell create a protein?
They use the ribosomes to connect strands from your DNA
How does the DNA work in cell cycles?
During interphase, the cell grows and makes a copy of its DNA. During the mitotic (M) phase, the cell separates its DNA into two sets and divides its cytoplasm, forming two new cells.
Why does a virus not have both DNA and RNA?
Because it doesn't use it's DNA or RNA to function, it uses it to inject into a cell and switch the cell's instructions to its own so the cell will make more viruses. Cells have DNA and RNA to tell the cell what to do, but viruses just do it naturally. Viruses have no use for both.
How does DNA in a new cell compare to the old cell when it replicates?
Since replication is semi-conservative and DNA replicates before mitosis the DNA in the new and old cell ( if you wish to use that terminology, as both are daughter cells ) is composed of one new strand and one old template strand.
