0
What else can I help you with?
Examples of intracellular enzymes?
Examples of intracellular enzymes include catalase, an enzyme that breaks down hydrogen peroxide in cells, and DNA polymerase, an enzyme involved in DNA replication. Additionally, enzymes like lipase and protease function intracellularly in breaking down lipid and protein molecules, respectively.
What reasoning led kary Mullis to search for DNA polymerase enzyme in bacteria living in the hot springs of Yellowstone national park?
Kary Mullis searched for DNA polymerase enzyme in bacteria from hot springs because the enzyme from typical sources was not heat-stable enough to be used in the polymerase chain reaction (PCR). He hypothesized that bacteria living in extreme environments, like the hot springs of Yellowstone, may have evolved more heat-stable enzymes that could withstand the high temperatures needed for PCR. This led him to discover the Taq polymerase, a key component in modern PCR technology.
What is the significance of thermostable polymerase in DNA technology?
Thermostable polymerase, like Taq polymerase, is important in DNA technology because it can withstand the high temperatures used in polymerase chain reaction (PCR). This allows for the rapid amplification of DNA fragments without the need to constantly replenish the enzyme. This polymerase is derived from thermophilic bacteria and is essential for the success of PCR in molecular biology applications.
Polymerase is considered what?
Polymerase is considered an enzyme that catalyzes the synthesis of nucleic acids, such as DNA and RNA, by assembling nucleotides into a polynucleotide chain. It plays a crucial role in processes like DNA replication and transcription, where it facilitates the copying of genetic information. Different types of polymerases exist, each specialized for specific tasks in the cell, such as DNA polymerase for DNA synthesis and RNA polymerase for RNA synthesis.
Does a nucleotide contain polymerase?
No; polymerase is a Protein that is made up from [or comprised of] Amino-Acids. While Amino-Acids make up - or comprise - proteins, nucleotides are the building 'blocks' for both DNA and Rna.
What enzyme catalyzes DNA replication?
one of them is heliocase. it 'unzips' the DNA strand. You can always remember this because it's in a popular joke: Q. Why is the enzyme heliocase a lot like a teenage boy? A. They both want to unzip your jeans (genes) !!!!!
What enzyme matches RNA nucleotides to complementary DNA nucleotides?
The enzyme that matches RNA nucleotides to complementary DNA nucleotides is called reverse transcriptase. It is used by retroviruses like HIV to convert their RNA genome into DNA before integrating it into the host cell's genome.
Does RNA polymerase require helicase for its function?
No, RNA polymerase does not require helicase for its function. RNA polymerase is responsible for synthesizing RNA from a DNA template, while helicase is an enzyme that unwinds the DNA double helix during processes like DNA replication and transcription.
Examples of intracellular enzymes?
Examples of intracellular enzymes include catalase, an enzyme that breaks down hydrogen peroxide in cells, and DNA polymerase, an enzyme involved in DNA replication. Additionally, enzymes like lipase and protease function intracellularly in breaking down lipid and protein molecules, respectively.
What reasoning led kary Mullis to search for DNA polymerase enzyme in bacteria living in the hot springs of Yellowstone national park?
Kary Mullis searched for DNA polymerase enzyme in bacteria from hot springs because the enzyme from typical sources was not heat-stable enough to be used in the polymerase chain reaction (PCR). He hypothesized that bacteria living in extreme environments, like the hot springs of Yellowstone, may have evolved more heat-stable enzymes that could withstand the high temperatures needed for PCR. This led him to discover the Taq polymerase, a key component in modern PCR technology.
What is the significance of thermostable polymerase in DNA technology?
Thermostable polymerase, like Taq polymerase, is important in DNA technology because it can withstand the high temperatures used in polymerase chain reaction (PCR). This allows for the rapid amplification of DNA fragments without the need to constantly replenish the enzyme. This polymerase is derived from thermophilic bacteria and is essential for the success of PCR in molecular biology applications.
Which enzyme is used specifically for rna transcription from dna in nucleus?
RNA polymerase.... What a wonderful, uplifting speech she gave at the convocation memorial service at Va Taech the day after the shootings there. She raised the spirits of both the students and the staff along with those of the world. She gave hope to the hopeless of the world and raised every single person involved with Va Tech to the highest of all highs. Va Tech is indeed lucky to have her as one of their professors. I hope and pray that they all recover from this horrible tragedy and with staff like her, the students will prevail! Go Hokies! Go Hokies!
How does PCR utilize DNA polymerase in the amplification process?
PCR, or polymerase chain reaction, uses DNA polymerase to amplify a specific region of DNA. The process involves heating the DNA to separate its two strands, then cooling it to allow primers to bind to the target sequence. DNA polymerase then extends the primers, creating new DNA strands that match the original sequence. This cycle is repeated multiple times, resulting in a significant increase in the amount of the target DNA.
Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
Does a nucleotide contain polymerase?
No; polymerase is a Protein that is made up from [or comprised of] Amino-Acids. While Amino-Acids make up - or comprise - proteins, nucleotides are the building 'blocks' for both DNA and Rna.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
What affect would temperature and pH have on the Catalans enzyme?
Just like always, deviating from the desired normal functioning for the enzyme, whether it be in temperature or pH, would result in the enzyme denaturing and therefore being unable to for enzyme substrate complexes, therefore reducing the overall reaction rate.
