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What is extracted from cells for DNA microarray technology?
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
What is the meaning of CDNA microarray?
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
How can you detect SNPs?
SNPs (single nucleotide polymorphisms) can be detected using various methods such as DNA sequencing, microarray analysis, and polymerase chain reaction (PCR) techniques. These methods can help to identify differences in the DNA sequence at a single nucleotide position among individuals.
What is Pros and cons of using DNA microarray?
I am a grad student taking a workshop in DNA microarray. I just started - so I am by no means an expert, but here is what I know so far. Pros Microarray technology is amazing in that it can be used to figure out which genes in an ENTIRE genome are upregulated or downregulated between two treatments. For example, if one treatment consisted of absolutely normal non cancerous prostate tissue, and the other treatment consisted of cancerous prostate tissue, you would be able to tell which genes are differentially expressed - an extremely powerful research tool. Cons The results cannot be taken as absolute fact, because they are partially a result of the manipulation of statistics. They must be corroborated afterwards with in vitro or in vivo experiments. It is REALLY expensive. Hope this helps!
What is the definition of genome analysis?
Genomic analysis are the techniques needed to determine and compare the genetic sequence (e.g. DNA in the chromosomes and mitochondria). This includes DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and the emergence of models of gene networks.
What is extracted from cells for DNA microarray technology?
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
What is the meaning of CDNA microarray?
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
3 Why should you normalize the micro array data?
Microarray data is usually applied for the comparison of gene expression profiles under different conditions. But we have to make sure what we are comparing is really comparable. So, to bring the data under common frame normalization is done. In technical terms, normalization of microarray data removes both random and systematic biases. [Source: Guide To Analysis of DNA Microarray Data]
What is the purpose of DNA microarray analysis?
Otherwise known as "microarray chips," DNA microarray are used to determined the genetic makeup of a given tissue sample. By shining various bands of light on these chips, the gene(s) in the tissue are expressed in the form of a particular color.
What are the pros and cons of integrating DNA microarray or chip technology into the forensic DNA laboratory?
Pros: High-throughput analysis: DNA microarrays or chips can analyze multiple DNA samples simultaneously, increasing efficiency. Increased information: Can provide information on multiple genetic markers, enabling more comprehensive analysis. Cost-effective: Allows for testing of multiple markers in a single assay, potentially reducing costs. Cons: Complexity: Requires specialized equipment and training, which may be costly and time-consuming to implement. Data interpretation: Results from microarray analysis may be complex and require specialized expertise for interpretation. Sensitivity: Microarray technology may have limitations in detecting low-level DNA samples compared to traditional DNA analysis methods.
What is DNA chip?
DNA chip or also called as DNA microarray is a collection of microscopic DNA spot attached to a firm surface. This is used by scientist to measure the expression level of the large amount of genes or to genotype numerous regions of a genome.
How can you detect SNPs?
SNPs (single nucleotide polymorphisms) can be detected using various methods such as DNA sequencing, microarray analysis, and polymerase chain reaction (PCR) techniques. These methods can help to identify differences in the DNA sequence at a single nucleotide position among individuals.
How does DNA microarray analysis for genetic screening impact the economy?
DNA microarray analysis allows both high throughput and high density analysis of genetic polymorphisms in humans and other types of organisms. It has significantly reduced the cost of scientific studies linking physical traits to specific genes, thus leading to a better understanding of the human body and what doctors can do to diagnose and treat diseases. Genetic analysis is a cost saving technology, in that it can be involved in preventative medicine as well as prescribing the best possible treatments and dosages for those diseases which have been well characterized. These effects can bring tremendous advantages in reducing the cost of care and thus have a beneficial effect on the economy.
What technique could tell you how gene expression differs between individuals with and without cystic fibrosis?
DNA Microarray
What is Pros and cons of using DNA microarray?
I am a grad student taking a workshop in DNA microarray. I just started - so I am by no means an expert, but here is what I know so far. Pros Microarray technology is amazing in that it can be used to figure out which genes in an ENTIRE genome are upregulated or downregulated between two treatments. For example, if one treatment consisted of absolutely normal non cancerous prostate tissue, and the other treatment consisted of cancerous prostate tissue, you would be able to tell which genes are differentially expressed - an extremely powerful research tool. Cons The results cannot be taken as absolute fact, because they are partially a result of the manipulation of statistics. They must be corroborated afterwards with in vitro or in vivo experiments. It is REALLY expensive. Hope this helps!
What is the definition of genome analysis?
Genomic analysis are the techniques needed to determine and compare the genetic sequence (e.g. DNA in the chromosomes and mitochondria). This includes DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and the emergence of models of gene networks.
What tools of recombinant DNA technology is incorrectly paired with its use?
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments
