DNA microarray analysis is a technique used to match up two strands of DNA. It is used in paternity tests and in criminal investigations in which a perpetrator's DNA was found at the crime scene.
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
SNPs (single nucleotide polymorphisms) can be detected using various methods such as DNA sequencing, microarray analysis, and polymerase chain reaction (PCR) techniques. These methods can help to identify differences in the DNA sequence at a single nucleotide position among individuals.
I am a grad student taking a workshop in DNA microarray. I just started - so I am by no means an expert, but here is what I know so far. Pros Microarray technology is amazing in that it can be used to figure out which genes in an ENTIRE genome are upregulated or downregulated between two treatments. For example, if one treatment consisted of absolutely normal non cancerous prostate tissue, and the other treatment consisted of cancerous prostate tissue, you would be able to tell which genes are differentially expressed - an extremely powerful research tool. Cons The results cannot be taken as absolute fact, because they are partially a result of the manipulation of statistics. They must be corroborated afterwards with in vitro or in vivo experiments. It is REALLY expensive. Hope this helps!
Genomic analysis are the techniques needed to determine and compare the genetic sequence (e.g. DNA in the chromosomes and mitochondria). This includes DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and the emergence of models of gene networks.
mRNA is extracted from cells for DNA microarray. the mRNA is then converted in the lab to cDNA this cDNA is allowed to interact with the probes on the microarray chip
A cDNA microarray is a hybrid of a DNA microarray, which is a collection of a number of minute DNA dots. These are mostly used in the field of genetic testing.
Microarray data is usually applied for the comparison of gene expression profiles under different conditions. But we have to make sure what we are comparing is really comparable. So, to bring the data under common frame normalization is done. In technical terms, normalization of microarray data removes both random and systematic biases. [Source: Guide To Analysis of DNA Microarray Data]
Otherwise known as "microarray chips," DNA microarray are used to determined the genetic makeup of a given tissue sample. By shining various bands of light on these chips, the gene(s) in the tissue are expressed in the form of a particular color.
Pros: High-throughput analysis: DNA microarrays or chips can analyze multiple DNA samples simultaneously, increasing efficiency. Increased information: Can provide information on multiple genetic markers, enabling more comprehensive analysis. Cost-effective: Allows for testing of multiple markers in a single assay, potentially reducing costs. Cons: Complexity: Requires specialized equipment and training, which may be costly and time-consuming to implement. Data interpretation: Results from microarray analysis may be complex and require specialized expertise for interpretation. Sensitivity: Microarray technology may have limitations in detecting low-level DNA samples compared to traditional DNA analysis methods.
DNA chip or also called as DNA microarray is a collection of microscopic DNA spot attached to a firm surface. This is used by scientist to measure the expression level of the large amount of genes or to genotype numerous regions of a genome.
SNPs (single nucleotide polymorphisms) can be detected using various methods such as DNA sequencing, microarray analysis, and polymerase chain reaction (PCR) techniques. These methods can help to identify differences in the DNA sequence at a single nucleotide position among individuals.
DNA microarray analysis allows both high throughput and high density analysis of genetic polymorphisms in humans and other types of organisms. It has significantly reduced the cost of scientific studies linking physical traits to specific genes, thus leading to a better understanding of the human body and what doctors can do to diagnose and treat diseases. Genetic analysis is a cost saving technology, in that it can be involved in preventative medicine as well as prescribing the best possible treatments and dosages for those diseases which have been well characterized. These effects can bring tremendous advantages in reducing the cost of care and thus have a beneficial effect on the economy.
DNA Microarray
I am a grad student taking a workshop in DNA microarray. I just started - so I am by no means an expert, but here is what I know so far. Pros Microarray technology is amazing in that it can be used to figure out which genes in an ENTIRE genome are upregulated or downregulated between two treatments. For example, if one treatment consisted of absolutely normal non cancerous prostate tissue, and the other treatment consisted of cancerous prostate tissue, you would be able to tell which genes are differentially expressed - an extremely powerful research tool. Cons The results cannot be taken as absolute fact, because they are partially a result of the manipulation of statistics. They must be corroborated afterwards with in vitro or in vivo experiments. It is REALLY expensive. Hope this helps!
Genomic analysis are the techniques needed to determine and compare the genetic sequence (e.g. DNA in the chromosomes and mitochondria). This includes DNA sequencing, routine use of DNA microarray technology for the analysis of gene expression profiles at the mRNA level and improved informatic tools to organize and analyze such data. At the same time, new developments in chip-based analysis of samples and the emergence of models of gene networks.
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments