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what antibody use in western blot technique?
Generally there are two antibodies used. Primary antibody which can bind specifically to the protein of interest. And a secondary antibody coupled with a detection system such as HRP that would bind the primary antibody and signals the presence of protein of interest.
What is primary and secondary antibody?
primary antibody is what binds to the specific gene that you are interested in looking at; i.e. primary is rabbit-antibody bind to its proper epitope. and this is usually unconjugated with no label. the secondary antibody is conjugated with some type of label, i.e., you will be able to see if your gene is being expressed. i.e., if primary from a rabbit, want goat-anti-rabbit, this way it can bind to the primary antibody.
What is a cold antibody?
A cold antibody is an antibody that reacts optimally at temperatures below body temperature, typically around 4°C. These antibodies can cause hemolysis (destruction of red blood cells) when blood is exposed to cold temperatures.
How many monomers make up an antibody molecule?
Four monomers. Each antibody contains 2 light chains and 2 heavy chains.
An antigen stimulates B cells to produce a specific what?
An antigen stimulates B cells to produce a specific antibody. This antibody is capable of recognizing and binding to the antigen that stimulated its production, marking it for destruction by other components of the immune system.
Is glycoprotein an antigen or antibody?
Antibody
What is a sentence using antibody?
the antibody can be uncontrollable
What part of speech is antibody?
Antibody is a noun.
What is the opposite word of antibody?
Antigen is the opposite of antibody.
What is the protein that destroys pathogen?
antibody
What does MAB mean?
Monoclonal antibody
Is an antibody a carbohydroate?
No, it is not. Antibody = A protein that fights infection.
Are chickenpox antibody and shingles antibody the same?
Chickenpox and shingles result from the same virus, and generate the same antibodies. There is no difference between chickenpox antibody and shingles antibody, and there is only one test (varicella virus antibody) for both.
A protein produced by the body to combat foreign particles?
Antibody
Is thyroglobulin antibody and anti thyroglobulin antibody same?
yes
Multiple antibody binding to single antigen?
Polyclonal antibody
Which antibody is present in sebum?
antibody IgA is found in sebum
