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"Most viable count" is a term used in microbiology to refer to the number of viable microorganisms in a sample. It is often determined through techniques like colony counting on agar plates or turbidity measurements. This count is important for assessing the presence and concentration of microorganisms in a sample.
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Does a spectrophotometer measure total cell count or viable cell count?
A spectrophotometer measures the optical density of a sample, which can be used to estimate total cell count in a sample. It does not distinguish between viable and non-viable cells, as both contribute to the absorption of light. To determine viable cell count, additional methods such as colony-forming unit assays or flow cytometry are typically used.
What is total viable count of bacteria?
Total viable count is a method used to estimate the total number of viable bacteria in a sample. This is typically done by plating a diluted sample onto an agar plate and counting the number of colonies that grow. It provides an estimate of the total number of bacteria that are able to grow and reproduce under the specific conditions used in the assay.
What is potential impact mutation?
No one knows what effect a mutation may have. Most are not viable.
What is affect of spores on tpc count?
Spores do not contribute to the total plate count (TPC) as they do not metabolize on standard culture media used for TPC determination. Only viable bacteria that can grow and form colonies will affect the TPC count. Spores may appear on plates as the result of sporulation by certain bacteria, but they are not included in the TPC calculation.
Why is the total viable count based on 30-300 colonies of microorganisms?
The range of 30-300 colonies is used because it provides a statistically significant sample size for counting colonies and estimating the number of viable microorganisms in a given sample. If there are too few colonies, the count may not be representative of the actual microbial population, and if there are too many colonies, it can be difficult to accurately count and differentiate individual colonies.
Does a spectrophotometer measure total cell count or viable cell count?
A spectrophotometer measures the optical density of a sample, which can be used to estimate total cell count in a sample. It does not distinguish between viable and non-viable cells, as both contribute to the absorption of light. To determine viable cell count, additional methods such as colony-forming unit assays or flow cytometry are typically used.
Name the units used for measuring micro-organisms.?
Total Viable Count,(TVC) or Total bacterial Count,(TBC)
WHY IS THE VIABLE PLATE COUNT TECHNIQUE CONSIDERED TO BE AN INDIRECT MEASUREMENT OF CELL DENSITY WHEREAS THE TURBIDIMETRY METHOD IS NOT COUNT AT ALL?
the viable plate technique refers to the number of viable cells in the plate. it called indirect method as some of cells that contribute to cell density may not viable. meanwhile turbidimetric method only count the cell density of microbial cell population, not count the number of cell. it monitored the changes of population by measuring the opacity of growth medium which will become more opaque as the cell numbers increase.
Why is the viable plate count technique considered to be an indirect measurement of cell density whereas the turbidimetry method is not a count at all?
the viable plate technique refers to the number of viable cells in the plate. it called indirect method as some of cells that contribute to cell density may not viable. meanwhile turbidimetric method only count the cell density of microbial cell population, not count the number of cell. it monitored the changes of population by measuring the opacity of growth medium which will become more opaque as the cell numbers increase.
Which fundamental assumption is the plate count method based on?
One major disadvantage of the viable plate count is the assumption that each colony arises from one cell.
Why direct microscopy and plate count give not a same counts?
Direct microscopy counts viable and non-viable cells, whereas plate count only counts viable cells that are able to grow and form colonies on agar plates. Additionally, plate count may underestimate the total number of viable cells due to factors like the inability of certain cell types to grow under specific conditions or the formation of aggregated cells that do not separate easily on the agar plate.
What is total viable count of bacteria?
Total viable count is a method used to estimate the total number of viable bacteria in a sample. This is typically done by plating a diluted sample onto an agar plate and counting the number of colonies that grow. It provides an estimate of the total number of bacteria that are able to grow and reproduce under the specific conditions used in the assay.
What does TVC stand for in meat?
TVC stands for Total Viable Count and is usually referred to when counting bacteria in meat.
Does the number of bacteria recorded by the standard plate count method accurately reflect the total bacterial count from the sample?
No. The standard plate count method is an indirect measurement of cell density of only viable bacterial cells. Optical density counting measure entire bacterial sample, the living as well as the dead bacterial cells.
How do you know if a mans sperm is good enough to make a baby?
It should always be considered viable. Even those with very low sperm count can have a number of viable sperm in their ejaculate. A fertility clinic or urologist can always run an analysis.
Will a cryptorchid dog produce viable sperm?
This will depend upon the exact state of the testicles. If both testicles are retained within the body wall, the dog will be infertile and produce no viable sperm. If only one testicle is retained within the body wall and the other has descended into the scrotum, the dog will produce viable sperm but likely have a low sperm count. If only one testicle is retained but it is outside the body wall although not in the scrotum, the dog will produce viable sperm and may have a normal sperm count. However, cryptorchidism (retained testicles) is a highly inherited condition, and it is likely that the dog would pass this defect on to his offspring. For this reason, most veterinarians and responsible breeders will advocate for the dog to be castrated and removed from the breeding pool.
What type of chromosomal abnormality is most likely to be viable in humans?
Trisomy
