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What is the purpose of sodium azide in the Bile Esculin test?
Sodium azide is included in the Bile Esculin test to inhibit the growth of Gram-negative organisms, which could interfere with the test results. Sodium azide helps to maintain a selective environment that favors the growth of group D streptococci, the target organisms for the test.
What ingredients of EMB agar inhibit the growth of gram positive organisms?
Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.
If you observed growth of gram negative organisms on the PEA plate you inoculated does the negate the usefulness of PEA as a selective medium why or why not?
Yes, the growth of gram-negative organisms on a PEA plate suggests that the medium is not effectively selective for gram-positive bacteria. PEA (Phenylethyl Alcohol Agar) is intended to inhibit gram-negative bacteria through the action of phenylethyl alcohol, so if gram-negative organisms are thriving, it indicates that their growth is not adequately suppressed. This could be due to several factors, including the specific strains or concentrations used, which may indicate limitations in PEA's selectivity under certain conditions.
What would prevent bacteria from moving between intestinal cells?
Bacteria movement between intestinal cells can be prevented by tight junctions, which are specialized structures that seal the spaces between epithelial cells, creating a barrier. Additionally, the presence of mucus and antimicrobial peptides in the intestinal lining can inhibit bacterial translocation. The immune response, including the secretion of immunoglobulins, also plays a key role in preventing bacterial movement and maintaining gut integrity.
Which nutrients enhances iron absorption from the intestinal tract?
Vitamin C is known to enhance iron absorption from the intestinal tract by promoting the conversion of non-heme iron (plant-based) to a form that is more easily absorbed by the body. Conversely, substances like tannins and phytates found in tea, coffee, and whole grains can inhibit iron absorption.
Dietary fiber seems to inhibit the development of colon cancer by?
Promoting movement of intestinal contents.
What is the purpose of sodium azide in the Bile Esculin test?
Sodium azide is included in the Bile Esculin test to inhibit the growth of Gram-negative organisms, which could interfere with the test results. Sodium azide helps to maintain a selective environment that favors the growth of group D streptococci, the target organisms for the test.
Why PEA contains 0.25 percent phenylethyl alcohol because high concentration inhibits both gram negative and gram positive?
At higher amounts, phenylethyl alcohol would most likely also inhibit gram positive organisms because it would be able to break down their membrane permeability barrier, thus allowing influx of substances ordinarily blocked and leakage of large amounts of cellular potassium. This ultimately disrupts or halts DNA synthesis.
What ingredients of EMB agar inhibit the growth of gram positive organisms?
Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.
If you observed growth of gram negative organisms on the PEA plate you inoculated does the negate the usefulness of PEA as a selective medium why or why not?
Yes, the growth of gram-negative organisms on a PEA plate suggests that the medium is not effectively selective for gram-positive bacteria. PEA (Phenylethyl Alcohol Agar) is intended to inhibit gram-negative bacteria through the action of phenylethyl alcohol, so if gram-negative organisms are thriving, it indicates that their growth is not adequately suppressed. This could be due to several factors, including the specific strains or concentrations used, which may indicate limitations in PEA's selectivity under certain conditions.
What chemical is produced by a living organism that kills or inhibits growth?
Antibiotics are chemicals produced by living organisms that kill or inhibit the growth of other organisms, particularly bacteria.
Do Organisms need proper temperature for growth and development?
Yes, organisms have an optimal temperature range for growth and development. Temperatures outside this range can slow down or inhibit these processes. Extreme temperatures can even be lethal to some organisms.
Do EMB plate dyes inhibit growth of Gram-positive or negative bac?
EMB plate dyes inhibit the growth of Gram-positive bacteria and promote the growth of Gram-negative bacteria. The dyes in EMB plates help differentiate between the two types of bacteria based on their ability to ferment lactose. Gram-negative bacteria that ferment lactose will produce dark colonies with a metallic green sheen.
What would prevent bacteria from moving between intestinal cells?
Bacteria movement between intestinal cells can be prevented by tight junctions, which are specialized structures that seal the spaces between epithelial cells, creating a barrier. Additionally, the presence of mucus and antimicrobial peptides in the intestinal lining can inhibit bacterial translocation. The immune response, including the secretion of immunoglobulins, also plays a key role in preventing bacterial movement and maintaining gut integrity.
What is the primary regulator of growth?
Genetics is the primary factor in an organism's ability to grow, although availability of nutrients can inhibit an organisms size as well.
What is the opposite of pheromones?
The opposite of pheromones would be substances that inhibit or mask the effects of pheromones, potentially disrupting communication or attraction signals between organisms.
How are antibiotics designed?
Antibiotics are not designed by any body. By defination they are synthesized by micro-organisms to inhibit growth of other micro-organisms. You have designing of the antibiotics in semisynthetic antibiotics. But probably it is done in trial and error method only, as on today.
