What is the definition of genomic footprinting?

Answer 1

Same as genetic footprinting, only at a high throughput, systems level way. Typically you use a transposon library to insert mutations to cells that inactivate genes (you assume one transposon/inactivation per cell). Then you trascription profile (dna microarray, rna-seq) after selection (whatever you want to select for, growth, resistance etc) and try to map the mutations/genes inactivations to the fitness changes in the cells.

For example if you select for growth in a particular environment and the deletion of a gene confers this, then during selection that mutant will be over-represented, so when you run a microarray, you will see no expression of the deleted gene in reference with the wild type strain.

Answer 2

Genomic footprinting is a technique used to identify regions of DNA that are bound by proteins, such as transcription factors. It involves digesting DNA that is bound to proteins, resulting in protected regions that can be detected. These protected regions represent the binding sites of proteins on the DNA.