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A high-performance liquid chromatography, or HPLC, refers to a technique in analytic chemistry that is used to separate the components in a mixture. The pump in HPLC passes a pressurized liquid solvent that contains the sample mixture through a column filled with a solid adsorbent material.

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Why ordinary liquid pump cannot be used in hplc system?

An ordinary liquid pump cannot be used in HPLC systems because HPLC requires pumps that can generate high pressure to push solvents through the column at a constant and precise flow rate. Ordinary pumps do not provide the level of pressure and flow rate control needed for HPLC separations, which can negatively impact the accuracy and reproducibility of chromatographic results. HPLC pumps are specifically designed to handle the high pressures and flow rates required for efficient separations.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


Is the protein pump active or passive in its function?

The protein pump is active in its function.


What are the HPLC Calibration parameters and elaborate it?

1. Flow rate 2. Temp. of column 3. Detector function 4. Resolution


Retention time calculation for hplc?

why RT was shifting & how to RT calculation in HPLC


How the hearts function?

PUMP


What type of species can be separated by HPLC but not by gas liquid chromatography?

mixture of enantiomers can be separated by HPLC


What is rs-hplc?

"RS-HPLC method" means "Related Substance HPLC Method".


Where can one purchase HPLC detectors?

You can purchase used HPLC detectors and other equipment from the usedhplc website or from the ebay bidding website. Alternatively you can buy HPLC detectors from the equipnet website.


What is RRT and RRF in hplc?

In HPLC RRT means Relative Retention Time and RRF is Relative Response Factor


What is dead volume HPLC?

The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.


What are the key differences between normal phase HPLC and reverse phase HPLC in terms of their separation mechanisms and applications?

Normal phase HPLC separates compounds based on their polarity, with the stationary phase being polar and the mobile phase being nonpolar. Reverse phase HPLC separates compounds based on their hydrophobicity, with the stationary phase being nonpolar and the mobile phase being polar. Normal phase HPLC is typically used for separating polar compounds, while reverse phase HPLC is used for separating nonpolar compounds.