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What concentration of alcohol is best to use for sterilization during plant tissue culture. 70 percent alcohol or absolute alcohol?
A concentration of 70% alcohol is generally preferred for sterilization during plant tissue culture. This is because a 70% solution is more effective at penetrating the cell walls of microorganisms, making it a better option for disinfection. Using absolute alcohol may be too harsh and could damage plant tissues.
Why sterile conditions are needed in tissue culture?
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
How do you avoid microbial contamination in plant tissue culture lab?
To avoid microbial contamination in a plant tissue culture lab, maintain strict aseptic techniques, ensure proper sterilization of equipment and growth media, maintain a clean work environment, and regularly monitor cultures for signs of contamination. Implementing a rigorous cleaning schedule and using fungicides or antibiotics when necessary can also help prevent microbial contamination.
What is the simple protocol for banana tissue culture?
in tissue culture should maintain some steps mandatory example explanting,surface sterilization, inoculation , incubation and subculture so their is no any simple protocol for tissue culture especially in banana need to take care still more than others plants because endogenous microbial contamination's will be more in succors so its better to follow specific protocol that should give healty plantlets free from diseases instead of going simple one.
When you look at the surface of your skin you see connective tissue?
The surface of your skin is primarily made up of epithelial tissue, not connective tissue. Connective tissue is found beneath the surface of the skin and plays a supportive role in providing structure and strength to the skin.
Are tissue culture and plant tissue culture same?
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
Dissertation topics in plant tissue culture?
tissue culture of mango se.
How can clone of a plant be produced?
by tissue culture method
What concentration of alcohol is best to use for sterilization during plant tissue culture. 70 percent alcohol or absolute alcohol?
A concentration of 70% alcohol is generally preferred for sterilization during plant tissue culture. This is because a 70% solution is more effective at penetrating the cell walls of microorganisms, making it a better option for disinfection. Using absolute alcohol may be too harsh and could damage plant tissues.
Why sterile conditions are needed in tissue culture?
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
What is difference between tissue culture cell culture and organ culture?
to culture any organ , tissue or cell from single cell called cell culture. to grow any tissue or organ from a tissue called tissue cultured. and formation of any organ from source organ is called organ culture. in short according to the source of culture any cultured are named.
What is the importance of tissue matching?
it is important because the wrong tissue can kill you
Why fresh mercuric chloride for tissue culture?
Usuallu 0.1% mercuric chloride is used for surface sterilization. Mercuric chloride reacs with ligth and will loose its activity if it is exposed to light, especially for a longer duration.. So usually fresh mercuric chloride is used (can be stored for a max of 1 or 2 weeks) and dont forget to store in amber coloured bottles, or cover the bottle
How do you avoid microbial contamination in plant tissue culture lab?
To avoid microbial contamination in a plant tissue culture lab, maintain strict aseptic techniques, ensure proper sterilization of equipment and growth media, maintain a clean work environment, and regularly monitor cultures for signs of contamination. Implementing a rigorous cleaning schedule and using fungicides or antibiotics when necessary can also help prevent microbial contamination.
Why the food making tissue is at the top surface of the leaf?
why is the food-making tissue at the leaf surface why
Advantages of tissue culture?
using tissue culture many plant can be grown from one parent in disease free condition
Which plants are most receptive to tissue culture?
Dicot plants are more receptive to tissue culture due to the presence of cambium.
