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Vitrification and Morphological Variations - Some shoots developed in vitro appear brittle, glassy and water-soaked; this is called vitrification or hyperhydracity.
In many species, vitrification may be represented by symptoms not visible to the naked eye, e.g., poorly developed vascular bundles, abnormal wax quality, abnormal functioning stomata, etc. Vitrification is the consequence of culture conditions, and leads to losses of plantlets.
It may be overcome by the following:
(i) increased agar levels (Ca. 1 %),
(ii) bottom cooling of culture vessels,
(iii) addition of agar hydrolysates, and
(iv) use of growth retardants.
Morphological variants may arise during the multiplication state. Such variants occur, in most cases, at a frequency comparable to that in vivo. But in commercial ventures using meristem cultures, visual selection is practised to eliminate variants and maintain the homogeneity of plantlets produced.
Many commercial enterprises, therefore, prefer to multiply shoots for only four or so cycles from an explant; after this, a fresh batch of cultures is initiated from field-tested plants.
What else can I help you with?
What is definition of ex-plant in plant tissue culture?
In plant tissue culture, ex-plant refers to the part of the plant that is used to initiate tissue culture. This can be any part of the plant, such as a leaf, stem, or root, that is sterilized and placed in a nutrient medium to grow into a new plant. The ex-plant serves as the starting material for tissue culture propagation.
What type of reproduction occurs in plant tissue culture?
Asexual Reproduction
What are the advantages of tissue culture?
Tissue culture allows for rapid multiplication of plant material, production of disease-free plants, and preservation of rare or endangered species. It also provides a controlled environment for studying plant growth and development.
What are the example of plant tissue culture?
plant tissue culture or cell culture:-it is the technique of in vitro, culture in which isolated plant cells, tissues, organs or even entire plant are subjected to grow in nutrient media in glass containers(tubes,conical flask,petri dishes) under aseptic conditions. Aseptic culture of plant may be of the following types as:-(a) ORGAN CULTURE:- These are cultures of isolated plant organs including cultures derived from root tips,stem tips,leaf primordia or immature part of flowers and immature fruits.(b) EMBRYO CULTURE:- These are culture of isolated immature or mature embryos.(c) CALLUS OR TISSUE CULTURE:- These are culture of tissue arising from disorganized proliferation of cells from segment of plant organs.tissue or callus culture are , generally grown on solid medium as amass of cells.(d) SUSPENSION CULTURE:- These are often called cell cultures, as they represent a lower level of organisation than tissue or callus culture. suspension culture are in vitro cultures of isolated cells and very small cell groups remaining dispersed as they grow in excited liquid media.
What are the functions of the plant tissue culture laboratory?
Plant tissue culture laboratories are used for propagation of plants on a large scale, conservation of rare and endangered plant species, production of disease-free plants, genetic transformation of plants, and research on plant growth and development. They play a crucial role in agriculture, horticulture, forestry, and conservation efforts by providing a controlled environment for the manipulation and multiplication of plant tissues.
Are tissue culture and plant tissue culture same?
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
Dissertation topics in plant tissue culture?
tissue culture of mango se.
How can clone of a plant be produced?
by tissue culture method
What is definition of ex-plant in plant tissue culture?
In plant tissue culture, ex-plant refers to the part of the plant that is used to initiate tissue culture. This can be any part of the plant, such as a leaf, stem, or root, that is sterilized and placed in a nutrient medium to grow into a new plant. The ex-plant serves as the starting material for tissue culture propagation.
What has the author Lii Jang Liu written?
Lii Jang Liu has written: 'Tropical plant cell and tissue culture' -- subject(s): Plant biotechnology, Plant cell culture, Plant micropropagation, Plant tissue culture
What has the author Roberta H Smith written?
Roberta H. Smith has written: 'Plant tissue culture' -- subject(s): Laboratory manuals, Plant tissue culture 'In Vitro Propagation of Kalanchoe (Avery's Plant Tissue Culture Series)'
Is plant tissue culture grown slowly or quickly?
Plant tissue culture usually takes some time to grow. Depending on the culture taken, it can take a couple weeks.
Advantages of tissue culture?
using tissue culture many plant can be grown from one parent in disease free condition
The process of growing a plant from a single cell or fragment from an adult plant?
plant tissue culture
What has the author S S Bhojwani written?
S. S. Bhojwani has written: 'The embryology of angiosperms' 'Plant tissue culture' -- subject(s): Plant tissue culture, Bibliography
What type of reproduction occurs in plant tissue culture?
Asexual Reproduction
What are the main function of the agar in the plant tissue culture medium?
Agar in plant tissue culture medium serves as a solidifying agent, providing a gel-like matrix for tissue support and growth. It also helps in the absorption and retention of water and nutrients by the plant tissues. Additionally, agar helps prevent contamination and provides a stable environment for tissue culture growth.
