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What are some ways chromatography can separate chemicals in a mixture?
Chromatography separates chemicals based on their affinity for a stationary phase and a mobile phase, allowing them to travel at different rates. Different types of chromatography like gas chromatography, liquid chromatography, and thin-layer chromatography utilize different mechanisms such as adsorption, partition, ion exchange, and size exclusion to separate the components in a mixture. By adjusting the conditions like solvent polarity, temperature, and column material, chromatography can effectively separate complex mixtures into individual components.
What are the different types of chromatography?
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
What is prime in ion chromatography?
In ion chromatography, "prime" typically refers to the process of preparing and conditioning the ion exchange column to ensure optimal performance. This involves flushing the column with a suitable eluent to remove impurities and equilibrate the stationary phase before sample analysis. Proper priming enhances resolution and sensitivity, allowing for accurate separation and quantification of ions in the sample.
What type of chromatography would be used to separate two proteins?
Size exclusion chromatography would be ideal for separating two proteins based on their size. This technique separates proteins by allowing smaller proteins to enter the pores of the stationary phase while larger proteins elute first.
What is the role of stationary phase in chromatographyh?
In chromatography, the stationary phase is a solid or liquid that remains fixed in place within the chromatography column or medium. Its primary role is to interact with the analytes as they pass through the system, allowing for the separation of components based on differences in their affinities for the stationary phase. This interaction can involve various forces, such as adsorption, partitioning, or ion exchange, which leads to varying retention times for different substances. Ultimately, the stationary phase is crucial for achieving efficient separation and resolution of the compounds being analyzed.
Is ion exchange adsorption and ion exchange chromatography is same?
Yes they are the same
What has the author Shahab A Shamsi written?
Shahab A Shamsi has written: 'Reversed phase /ion chromatography and capillary electrophoresis of ionic compounds with indirect detection' -- subject(s): Chemistry, Ion exchange chromatography, Capillary electrophoresis
What are some ways chromatography can separate chemicals in a mixture?
Chromatography separates chemicals based on their affinity for a stationary phase and a mobile phase, allowing them to travel at different rates. Different types of chromatography like gas chromatography, liquid chromatography, and thin-layer chromatography utilize different mechanisms such as adsorption, partition, ion exchange, and size exclusion to separate the components in a mixture. By adjusting the conditions like solvent polarity, temperature, and column material, chromatography can effectively separate complex mixtures into individual components.
What are the different types of chromatography?
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
What are the key differences between anion exchange chromatography and cation exchange chromatography?
Anion exchange chromatography and cation exchange chromatography are both types of ion exchange chromatography used to separate molecules based on their charge. The key difference between them is the type of ions they attract and retain. Anion exchange chromatography attracts and retains negatively charged ions (anions), while cation exchange chromatography attracts and retains positively charged ions (cations).
What are separation technique of solutions?
Examples: distillation, ion-exchange, chromatography, decantation, extraction.
What is prime in ion chromatography?
In ion chromatography, "prime" typically refers to the process of preparing and conditioning the ion exchange column to ensure optimal performance. This involves flushing the column with a suitable eluent to remove impurities and equilibrate the stationary phase before sample analysis. Proper priming enhances resolution and sensitivity, allowing for accurate separation and quantification of ions in the sample.
What has the author Joseph X Khym written?
Joseph X. Khym has written: 'Analytical ion-exchange procedures in chemistry and biology: theory, equipment, techniques' -- subject(s): Ion exchange, Ion exchange chromatography
What has the author Douglas T Gjerde written?
Douglas T. Gjerde has written: 'Ion chromatography' -- subject(s): Ion exchange chromatography 'DNA chromatography' -- subject(s): Analysis, Chromatographic analysis, DNA, Genetic Techniques, High Pressure Liquid Chromatography, Isolation & purification
Why do phosphate ions elute before sulfate ions in ion chromatography?
They can actually elute in either order. It depends on your mobile phase. In a weakly basic solution, the phosphate ions are found more as HPO4 (2-) rather than PO4 (3-) and thus will elute before sulfate which is larger and has the same charge. In a strongly basic mobile phase, the PO4 (3-) ion will dominate, and will elute after the sulfate ion
What has the author T R Acciani written?
T. R Acciani has written: 'Chemical analysis of wet scrubbers utilizing ion chromatography' -- subject(s): Analysis, Ion exchange chromatography, Scrubber (Chemical technology)
What type of chromatography would be used to separate two proteins?
Size exclusion chromatography would be ideal for separating two proteins based on their size. This technique separates proteins by allowing smaller proteins to enter the pores of the stationary phase while larger proteins elute first.
