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What else can I help you with?
What is commonly found in a cell membrane?
Peptidoglycan, being the most important element for gram staining, cell differentiation....etc...
What is the importance of gram staining?
Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. It is a first step to determine the identity of a particular bacterial sample. Gram stains are performed on body fluid or biopsy when infection is suspected. It yields results much more quickly than culture, and is especially important when infection would make an important difference in the patient's treatment and prognosis.
What happens if you use gram stain on a virus?
Most likely nothing. The gram stain will only stain the peptidoglycan cell wall of bacteria. Most viruses have a protein coat called a capsid. The capsid will not take up either the crystal violet or the safranin of a Gram stain.
Why do physician perform a gram stain on a sample before prescribing an antibiotic?
In order to know whose the best antibiotic to be prescribed. There are some antibiotics that are specific of a gram type (they won't be useful on both types), while there are other antibiotics that have a greater effect on a specific Gram type than on the other one.
Most pathogenic bacteria are consumer eubacteria and are grouped according to what characteristic?
Most pathogenic bacteria, which are classified as consumer eubacteria, are grouped primarily based on their shape (morphology), staining properties (Gram-positive or Gram-negative), and metabolic characteristics. These classifications help in identifying the bacteria and determining their potential effects on human health. Additional factors, such as oxygen requirements and biochemical properties, are also considered in their classification.
What is commonly found in a cell membrane?
Peptidoglycan, being the most important element for gram staining, cell differentiation....etc...
Which step is most crucial or most likely to cause poor results in the gram stain?
Gram staining is a type of differential staining in which two types of bacteria are differentiated on the basis of their cell wall either gram positive or gram negative although all the steps in gram staining are crucial, the most important step the most crucial step in the performance of the Gram staining procedure is the decolorization step which is the Acid-Alcohol (3% HCl and 95% Ethanol) and must be timed correctly; the crystal violet stain will be removed from both Gram-positive and negative cells if the decolorizing agent is left on too long (a matter of seconds).
Why must young cultures be used when doing staining?
Young cultures must be used when doing a gram stain to get more accurate results. The cell was is the part of the bacterial cell that is most involved with gram staining because it holds the crystal violet.
If at the end of gram staining both the gram positive and gram negative bacteria appear red what is the most likely explanation?
If both gram positive and gram negative bacteria appear red after Gram staining, it is likely that either the staining process was not performed correctly or the decolorization step was not carried out properly. This could lead to both types of bacteria retaining the red stain (crystal violet) and not taking up the counterstain (safranin). Checking the procedure and ensuring proper timing of each step can help to correct this issue.
What is the importance of gram staining?
Gram staining (or Gram's method) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. It is a first step to determine the identity of a particular bacterial sample. Gram stains are performed on body fluid or biopsy when infection is suspected. It yields results much more quickly than culture, and is especially important when infection would make an important difference in the patient's treatment and prognosis.
What happens if you use gram stain on a virus?
Most likely nothing. The gram stain will only stain the peptidoglycan cell wall of bacteria. Most viruses have a protein coat called a capsid. The capsid will not take up either the crystal violet or the safranin of a Gram stain.
What part of bacterial cell is most involve with gram staining and why?
In the gram staining process, gram positive bacteria appear to be purple because their cell walls, which contain a large concentration of peptidoglycans, are strongly dyed. Gram negative bacteria appear pink because their walls asborb less dye. This occurs becayse there is a smaller concentration of peptidoglycans and an additional lipid layer surrounds the cell wall. ANSWER The exact mechanism of action of this staining technique is not clearly understood. However, it is known that differences in the biochemical composition of bacterial cell walls parallel differences in their Gram-stain reactions. Gram-positive bacterial walls are rich in tightly linked peptidoglycans (protein-sugar complexes) that enable cells to resist decolorization. Gram-negative bacterial walls have a high concentration of lipids (fats) that dissolve in the decolorizer (alcohol, acetone, or a mixture of these) and are washed away with the crystal violet. The decolorizer thus prepares gram-negative organisms for the counterstain.
What is the most common stain?
The most common stain on clothes is probably food stains, such as grease, tomato sauce, or chocolate. These stains can be stubborn to remove if not treated promptly.
Why must fresh bacterial cultures be used in a Gram stain?
Why must young cultures be used when doing a Gram stain Young cultures must be used so the crystal violet can stick to the cell walls of Gram positive bacteria. The cell walls break down in old cultures and the staining process is not accurate
Why are the steps in gram staining so carefully standardized?
Gram's stain remains one of the most valuable methods we have for identifying isolates accurately and rapidly. Despite our long-standing familiarity with this method, it still warrants careful attention every step of the way--from preparation and QC of reagents to staining and interpretation. I think one of the main reasons would to avoid contamination.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
