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How many bases of DNA are in 1 kilobase fragment?
There are 1000 bases of DNA in a 1 kilobase fragment.
Is genetic egineering the insertion of a small fragment of frog DNA into the DNA of another species?
Genetic engineering
What is semidiscontinuous DNA means?
Semidiscontinuous DNA is a lagging strand synthesis. It is a short fragment in the structure of double helix.
What general shape does this fragment of DNA resemble?
The DNA molecules resembles a twisted step ladder
Why might a laboratory be using dideoxy nucleotides?
A laboratory might be using dideoxy nucleotides in DNA sequencing reactions, specifically in the Sanger method. Dideoxy nucleotides lack a 3’ hydroxyl group needed for DNA chain elongation, resulting in termination of DNA synthesis at specific bases. This allows for the determination of the nucleotide sequence of a DNA fragment.
How many bases of DNA are in 1 kilobase fragment?
There are 1000 bases of DNA in a 1 kilobase fragment.
Which fragment of DNA is the longest or biggest?
The fragment of the DNA that is the longest is the one that is the slowest to get to the bottom of the gel in the body. This is because longer DNA are simply the largest base pairs that are digested in the restriction enzymes which make them slower then the shorter ones.
An okazaki fragment has which of the following arrangements?
An Okazaki fragment is a short, newly synthesized DNA fragment that is formed on the lagging strand during DNA replication. It is composed of a short RNA primer at the 5' end and DNA nucleotides that extend toward the replication fork.
Is genetic egineering the insertion of a small fragment of frog DNA into the DNA of another species?
Genetic engineering
What is a vector used for in a genetic engineering experiment?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
Why does the DNA fragment size change as you move from the cathode to the anode?
In gel electrophoresis, DNA fragments move towards the anode (positive electrode) because DNA is negatively charged. Smaller fragments move faster through the gel matrix, so they appear closer to the anode while larger fragments move slower and appear closer to the cathode. This results in separation of DNA fragments based on size.
What is true of the DNA fragment band closest to the positive end of the gel?
they are the smallest.
What is semidiscontinuous DNA means?
Semidiscontinuous DNA is a lagging strand synthesis. It is a short fragment in the structure of double helix.
The sequence of a DNA fragment can be determined by observing the order of the fluorescent bands on the electrophoresis gel?
Yes, the sequence of a DNA fragment can be determined by the order of the fluorescent bands on the electrophoresis gel, which corresponds to the sequence of the nucleotides in the fragment. Each fluorescent band represents a different nucleotide in the DNA sequence. By comparing the band pattern to a known sequence ladder, the sequence of the DNA fragment can be read.
What general shape does this fragment of DNA resemble?
The DNA molecules resembles a twisted step ladder
A dna fragment with a sticky end that reads -attcg will bind with another dna fragment with a sticky end that reads?
ATTCG signify adenine (A), thymine (T), cytosine (C) and guanine (G). The bonding pairs are AT, GC when DNA replicates. Therefore the fragment ATTCG will bind to TAAGC.
Why might a laboratory be using dideoxy nucleotides?
A laboratory might be using dideoxy nucleotides in DNA sequencing reactions, specifically in the Sanger method. Dideoxy nucleotides lack a 3’ hydroxyl group needed for DNA chain elongation, resulting in termination of DNA synthesis at specific bases. This allows for the determination of the nucleotide sequence of a DNA fragment.
