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DNA fragment who are protected by a DNA binding protein are not digest. one can see the sequence recongnition
wow.....what an answer?!
DnaseI digests DNA nondiscriminantly, but at low concentrations the DNaseI nuclease cannot digest as efficiently. Only the loosely packaged chromatin is digested. this is a method used to study chromatin (DNA) packaging. Sensitivity (Hypersensitivity) to DNaseI is usually associated with actively transcribed genes, where regulatory elements are accessible to regulatory proteins. baculoboy
What else can I help you with?
What is the reagent used to test Dnase test?
There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.
Describe a positive DNase test?
A positive DNase test indicates the presence of DNase enzyme, which degrades DNA molecules. This is detected by a clear zone around the bacterial growth on DNase agar plate after adding a DNase indicator dye. The clear zone indicates that DNA in the medium has been hydrolyzed, suggesting the bacteria has the ability to produce DNase enzyme.
Is serratia marcescens dnase positive or negative?
Serratia genus as a whole is dnase positive.
Does DNase affect RNA?
Yes, DNase can affect RNA because it specifically targets and degrades DNA molecules. If RNA is contaminated with DNA, DNase treatment can help remove the DNA, but it will not affect the RNA molecules themselves.
Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
What is the reagent used to test Dnase test?
There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.
Describe a positive DNase test?
A positive DNase test indicates the presence of DNase enzyme, which degrades DNA molecules. This is detected by a clear zone around the bacterial growth on DNase agar plate after adding a DNase indicator dye. The clear zone indicates that DNA in the medium has been hydrolyzed, suggesting the bacteria has the ability to produce DNase enzyme.
Why is the DNase test only incubated for 24 hrs?
The DNase test is incubated for 24 hours because this is enough time for the enzyme DNase to degrade any DNA present in the agar. Prolonged incubation can lead to false negative results due to the breakdown of the DNA substrate.
What is the purpose of the anti-DNase-B test?
The anti-DNase-B (ADB) test is performed to determine a previous infection of a specific type of Streptococcus, group A beta-hemolytic Streptococcus.
Does m luteus produce a positive or negative dnase test?
yes
Why 1M of HCL is used in Dnase test?
Hydrochloric acid (HCl) is used in DNase tests to denature proteins that may inhibit the enzyme DNase. By denaturing proteins, the HCl helps to create an environment that is more conducive for the DNase enzyme to work effectively in degrading DNA. Using 1M of HCl provides an optimal concentration for denaturing proteins without affecting the stability and activity of DNase.
Which type of enzyme destroys the ability of the transforming principle to function?
DNase enzyme destroys the ability of the transforming principle to function by degrading DNA molecules.
Is serratia marcescens dnase positive or negative?
Serratia genus as a whole is dnase positive.
Does human hands contain DNAse enzyme?
Yes, human hands contain DNAse enzymes. DNAse enzymes are involved in breaking down and degrading DNA molecules. In the human body, DNAse enzymes can be found in various tissues and cells, including skin cells in the hands.
What is an anti-DNase-B test?
detects antigens produced by group A strep, and is elevated in most patients with rheumatic fever and poststreptococcal glomerulonephritis.
Does DNase affect RNA?
Yes, DNase can affect RNA because it specifically targets and degrades DNA molecules. If RNA is contaminated with DNA, DNase treatment can help remove the DNA, but it will not affect the RNA molecules themselves.
Principle of widal test?
same principle for latex methd
